Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium
Аутори
Popović, Nataša M.Stojiljković, Vesna
Pejić, Snežana
Todorović, Ana
Pavlović, Ivan
Gavrilović, Ljubica
Pajović, Snežana B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lit...hium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.
Извор:
Oxidative Medicine and Cellular Longevity, 2019, 2019, 1-11Финансирање / пројекти:
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Молекуларно физиолошки биомониторинг аеробних организама заснован на одређивању биохемијских биомаркера оксидационог стреса (RS-MESTD-Basic Research (BR or ON)-173041)
- Акутни коронарни синдром: истраживање вулнерабилности (плака, крви и миокарда), оптимално лечење и одређивање прогностичких фактора (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41022)
DOI: 10.1155/2019/8745376
ISSN: 1942-0900; 1942-0994
PubMed: 30911352
WoS: 000460272000001
Scopus: 2-s2.0-85062565744
URI
https://www.hindawi.com/journals/omcl/2019/8745376/https://vinar.vin.bg.ac.rs/handle/123456789/8085
Институција/група
VinčaTY - JOUR AU - Popović, Nataša M. AU - Stojiljković, Vesna AU - Pejić, Snežana AU - Todorović, Ana AU - Pavlović, Ivan AU - Gavrilović, Ljubica AU - Pajović, Snežana B. PY - 2019 UR - https://www.hindawi.com/journals/omcl/2019/8745376/ UR - https://vinar.vin.bg.ac.rs/handle/123456789/8085 AB - This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions. T2 - Oxidative Medicine and Cellular Longevity T1 - Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium VL - 2019 SP - 1 EP - 11 DO - 10.1155/2019/8745376 ER -
@article{ author = "Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica and Pajović, Snežana B.", year = "2019", abstract = "This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.", journal = "Oxidative Medicine and Cellular Longevity", title = "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium", volume = "2019", pages = "1-11", doi = "10.1155/2019/8745376" }
Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Gavrilović, L.,& Pajović, S. B.. (2019). Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity, 2019, 1-11. https://doi.org/10.1155/2019/8745376
Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L, Pajović SB. Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity. 2019;2019:1-11. doi:10.1155/2019/8745376 .
Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Gavrilović, Ljubica, Pajović, Snežana B., "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium" in Oxidative Medicine and Cellular Longevity, 2019 (2019):1-11, https://doi.org/10.1155/2019/8745376 . .