Effect of magnetic nanoparticles coating on cell proliferation and uptake
Само за регистроване кориснике
2019
Аутори
Zavišova, VlastaKoneracka, Martina
Gabelova, Alena
Svitkova, Barbora
Ursinyova, Monika
Kubovčikova, Martina
Antal, Iryna
Khmara, Iryna
Jurikova, Alena
Molčan, Matuš
Ognjanović, Miloš
Antić, Bratislav
Kopčansky, Peter
Чланак у часопису (Објављена верзија)
,
© 2018 Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт
Magnetic iron oxide nanoparticles (MNPs) are one of the most promising types of nanoparticles for biomedical applications, primarily in the context of nanomedicine-based diagnostics and therapy. They are used as contrast agents in magnetic resonance imaging and magnetite cell labelling. Furthermore, they are promising heating mediator in magnetic hyperthermia-based therapy, and can serve as nanocarriers in targeted gene and drug delivery as well. In biomedical applications, coating plays an important role in nanoparticle dispersion stability and biocompatibility. However, the impact of nanoparticle surface chemistry on cell uptake and proliferation has not been sufficiently investigated. The objective of this study is to prepare magnetic nanoparticles with inner magnetite core and hydrophilic outer shell of surfactant, protein and polymers that are commonly used in biomedical research. MNPs were characterized in-depth by various physicochemical methods. Magnetic hyperthermia, applied t...o find out the influence of MNPs coating on heating characteristics of the samples, did not show any correlation between layer thickness and specific adsorption rate. To evaluate the impact of surface chemistry on cell proliferation and internalization, the human lung adenocarcinoma epithelial (A549) cells were utilized. Substantial differences were determined in the amount of internalized MNPs and cell viability in dependence on surface coating. Our results indicate that the surface chemistry not only protects particles from agglomeration but also affect the interaction between cell and MNPs. © 2018 Elsevier B.V.
Кључне речи:
Magnetic nanoparticles / Magnetic fluid / Magnetic hyperthermia / Cytotoxicity / Cell uptakeИзвор:
Journal of Magnetism and Magnetic Materials, 2019, 472, 66-73Финансирање / пројекти:
- Slovak Research and Development Agency (APVV-14-0120)
- Slovak Research and Development Agency (APVV-14-0932)
- Slovak Research and Development Agency (APVV-15-0453)
- Scientific Grant Agency (2/0056/17)
- Scientific Grant Agency (2/0113/15)
- Scientific Grant Agency (2/0016/17)
- Scientific Grant Agency (2/0141/16)
- PROMATECH (ITMS No. 26220220186)
- Center of excellence of environmental health (ITMS No. 26240120033)
- H2020 (H2020/2014-2020) funding under Grant agreement 685817 – HISENTS
- EEA FM and the NFM (Project SK0020)
- COST action TD1402 (RADIOMAG)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
DOI: 10.1016/j.jmmm.2018.09.116
ISSN: 0304-8853
WoS: 000449681300012
Scopus: 2-s2.0-85054580890
URI
https://linkinghub.elsevier.com/retrieve/pii/S0304885318320183https://vinar.vin.bg.ac.rs/handle/123456789/7906
Колекције
Институција/група
VinčaTY - JOUR AU - Zavišova, Vlasta AU - Koneracka, Martina AU - Gabelova, Alena AU - Svitkova, Barbora AU - Ursinyova, Monika AU - Kubovčikova, Martina AU - Antal, Iryna AU - Khmara, Iryna AU - Jurikova, Alena AU - Molčan, Matuš AU - Ognjanović, Miloš AU - Antić, Bratislav AU - Kopčansky, Peter PY - 2019 UR - https://linkinghub.elsevier.com/retrieve/pii/S0304885318320183 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7906 AB - Magnetic iron oxide nanoparticles (MNPs) are one of the most promising types of nanoparticles for biomedical applications, primarily in the context of nanomedicine-based diagnostics and therapy. They are used as contrast agents in magnetic resonance imaging and magnetite cell labelling. Furthermore, they are promising heating mediator in magnetic hyperthermia-based therapy, and can serve as nanocarriers in targeted gene and drug delivery as well. In biomedical applications, coating plays an important role in nanoparticle dispersion stability and biocompatibility. However, the impact of nanoparticle surface chemistry on cell uptake and proliferation has not been sufficiently investigated. The objective of this study is to prepare magnetic nanoparticles with inner magnetite core and hydrophilic outer shell of surfactant, protein and polymers that are commonly used in biomedical research. MNPs were characterized in-depth by various physicochemical methods. Magnetic hyperthermia, applied to find out the influence of MNPs coating on heating characteristics of the samples, did not show any correlation between layer thickness and specific adsorption rate. To evaluate the impact of surface chemistry on cell proliferation and internalization, the human lung adenocarcinoma epithelial (A549) cells were utilized. Substantial differences were determined in the amount of internalized MNPs and cell viability in dependence on surface coating. Our results indicate that the surface chemistry not only protects particles from agglomeration but also affect the interaction between cell and MNPs. © 2018 Elsevier B.V. T2 - Journal of Magnetism and Magnetic Materials T1 - Effect of magnetic nanoparticles coating on cell proliferation and uptake VL - 472 SP - 66 EP - 73 DO - 10.1016/j.jmmm.2018.09.116 ER -
@article{ author = "Zavišova, Vlasta and Koneracka, Martina and Gabelova, Alena and Svitkova, Barbora and Ursinyova, Monika and Kubovčikova, Martina and Antal, Iryna and Khmara, Iryna and Jurikova, Alena and Molčan, Matuš and Ognjanović, Miloš and Antić, Bratislav and Kopčansky, Peter", year = "2019", abstract = "Magnetic iron oxide nanoparticles (MNPs) are one of the most promising types of nanoparticles for biomedical applications, primarily in the context of nanomedicine-based diagnostics and therapy. They are used as contrast agents in magnetic resonance imaging and magnetite cell labelling. Furthermore, they are promising heating mediator in magnetic hyperthermia-based therapy, and can serve as nanocarriers in targeted gene and drug delivery as well. In biomedical applications, coating plays an important role in nanoparticle dispersion stability and biocompatibility. However, the impact of nanoparticle surface chemistry on cell uptake and proliferation has not been sufficiently investigated. The objective of this study is to prepare magnetic nanoparticles with inner magnetite core and hydrophilic outer shell of surfactant, protein and polymers that are commonly used in biomedical research. MNPs were characterized in-depth by various physicochemical methods. Magnetic hyperthermia, applied to find out the influence of MNPs coating on heating characteristics of the samples, did not show any correlation between layer thickness and specific adsorption rate. To evaluate the impact of surface chemistry on cell proliferation and internalization, the human lung adenocarcinoma epithelial (A549) cells were utilized. Substantial differences were determined in the amount of internalized MNPs and cell viability in dependence on surface coating. Our results indicate that the surface chemistry not only protects particles from agglomeration but also affect the interaction between cell and MNPs. © 2018 Elsevier B.V.", journal = "Journal of Magnetism and Magnetic Materials", title = "Effect of magnetic nanoparticles coating on cell proliferation and uptake", volume = "472", pages = "66-73", doi = "10.1016/j.jmmm.2018.09.116" }
Zavišova, V., Koneracka, M., Gabelova, A., Svitkova, B., Ursinyova, M., Kubovčikova, M., Antal, I., Khmara, I., Jurikova, A., Molčan, M., Ognjanović, M., Antić, B.,& Kopčansky, P.. (2019). Effect of magnetic nanoparticles coating on cell proliferation and uptake. in Journal of Magnetism and Magnetic Materials, 472, 66-73. https://doi.org/10.1016/j.jmmm.2018.09.116
Zavišova V, Koneracka M, Gabelova A, Svitkova B, Ursinyova M, Kubovčikova M, Antal I, Khmara I, Jurikova A, Molčan M, Ognjanović M, Antić B, Kopčansky P. Effect of magnetic nanoparticles coating on cell proliferation and uptake. in Journal of Magnetism and Magnetic Materials. 2019;472:66-73. doi:10.1016/j.jmmm.2018.09.116 .
Zavišova, Vlasta, Koneracka, Martina, Gabelova, Alena, Svitkova, Barbora, Ursinyova, Monika, Kubovčikova, Martina, Antal, Iryna, Khmara, Iryna, Jurikova, Alena, Molčan, Matuš, Ognjanović, Miloš, Antić, Bratislav, Kopčansky, Peter, "Effect of magnetic nanoparticles coating on cell proliferation and uptake" in Journal of Magnetism and Magnetic Materials, 472 (2019):66-73, https://doi.org/10.1016/j.jmmm.2018.09.116 . .