Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine
Authors
Đorđević, Neda O.Todorović, Nevena
Novaković, Irena T.
Pezo, Lato
Pejin, Boris
Maraš, Vesna
Tešević, Vele V.
Pajović, Snežana B.
Article (Published version)
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Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2–stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2–stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2–stressed cells and shifted antioxida...tive defense towards GPx–mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.
Keywords:
red wine / polyphenolics / antioxidant activity / Saccharomyces cerevisiae / artificial neural networkSource:
Molecules, 2018, 23, 8, 1971-Funding / projects:
- Cellular and molecular basis of malignant and cardiovascular diseases-clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.3390/molecules23081971
ISSN: 1420-3049
PubMed: 30087228
WoS: 000445295500136
Scopus: 2-s2.0-85052383450
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VinčaTY - JOUR AU - Đorđević, Neda O. AU - Todorović, Nevena AU - Novaković, Irena T. AU - Pezo, Lato AU - Pejin, Boris AU - Maraš, Vesna AU - Tešević, Vele V. AU - Pajović, Snežana B. PY - 2018 UR - http://www.mdpi.com/1420-3049/23/8/1971 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7858 AB - Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2–stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2–stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2–stressed cells and shifted antioxidative defense towards GPx–mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system. T2 - Molecules T1 - Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine VL - 23 IS - 8 SP - 1971 DO - 10.3390/molecules23081971 ER -
@article{ author = "Đorđević, Neda O. and Todorović, Nevena and Novaković, Irena T. and Pezo, Lato and Pejin, Boris and Maraš, Vesna and Tešević, Vele V. and Pajović, Snežana B.", year = "2018", abstract = "Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2–stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2–stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2–stressed cells and shifted antioxidative defense towards GPx–mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.", journal = "Molecules", title = "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine", volume = "23", number = "8", pages = "1971", doi = "10.3390/molecules23081971" }
Đorđević, N. O., Todorović, N., Novaković, I. T., Pezo, L., Pejin, B., Maraš, V., Tešević, V. V.,& Pajović, S. B.. (2018). Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules, 23(8), 1971. https://doi.org/10.3390/molecules23081971
Đorđević NO, Todorović N, Novaković IT, Pezo L, Pejin B, Maraš V, Tešević VV, Pajović SB. Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules. 2018;23(8):1971. doi:10.3390/molecules23081971 .
Đorđević, Neda O., Todorović, Nevena, Novaković, Irena T., Pezo, Lato, Pejin, Boris, Maraš, Vesna, Tešević, Vele V., Pajović, Snežana B., "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine" in Molecules, 23, no. 8 (2018):1971, https://doi.org/10.3390/molecules23081971 . .