MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
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Аутори
Radović, NikolaNikolić Jakoba, Nataša
Petrović, Nina
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Milosavljević, Aleksandra
Brković, Božidar
Roganović, Jelena
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© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
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Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR...-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
Кључне речи:
diabetes mellitus type 2 / epigenetic modulators / miR-146a / miR-155 / periodontitisИзвор:
Journal of Clinical Periodontology, 2018, 45, 6, 663-671Финансирање / пројекти:
- Контрола бола и молекуларни механизми као фактори регенеративне терапије у стоматологији код здравих и пацијената са дијабетес мелитусом (RS-MESTD-Basic Research (BR or ON)-175021)
DOI: 10.1111/jcpe.12888
ISSN: 0303-6979; 1600-051X
PubMed: 29517812
WoS: 000434128500003
Scopus: 2-s2.0-85047460731
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Институција/група
VinčaTY - JOUR AU - Radović, Nikola AU - Nikolić Jakoba, Nataša AU - Petrović, Nina AU - Milosavljević, Aleksandra AU - Brković, Božidar AU - Roganović, Jelena PY - 2018 UR - http://doi.wiley.com/10.1111/jcpe.12888 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7753 AB - Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. T2 - Journal of Clinical Periodontology T1 - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients VL - 45 IS - 6 SP - 663 EP - 671 DO - 10.1111/jcpe.12888 ER -
@article{ author = "Radović, Nikola and Nikolić Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena", year = "2018", abstract = "Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.", journal = "Journal of Clinical Periodontology", title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients", volume = "45", number = "6", pages = "663-671", doi = "10.1111/jcpe.12888" }
Radović, N., Nikolić Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology, 45(6), 663-671. https://doi.org/10.1111/jcpe.12888
Radović N, Nikolić Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671. doi:10.1111/jcpe.12888 .
Radović, Nikola, Nikolić Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671, https://doi.org/10.1111/jcpe.12888 . .