In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
Nema prikaza
Autori
Ignjatović, Nenad L.Ninkov, Petar
Sabetrasekh, Roya
Lyngstadaas, Staale Petter
Uskoković, Dragan
Članak u časopisu (Objavljena verzija)
,
© 2014 - IOS Press and the authors
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional ...drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
Ključne reči:
calcium phosphate / drug delivery / nanomaterials / poly-DL-lactide-co-glycolideIzvor:
Bio-Medical Materials and Engineering, 2014, 1647-1658Finansiranje / projekti:
- Molekularno dizajniranje nanočestica kontrolisanih morfoloških i fizičko-hemijskih karakteristika i funkcionalnih materijala na njihovoj osnovi (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
Napomena:
- Peer-reviewed version of the article (Accepted Manuscript or postprint) available at: https://vinar.vin.bg.ac.rs/handle/123456789/7475
DOI: 10.3233/BME-140978
ISSN: 0959-2989
WoS: 000337913300001
Scopus: 2-s2.0-84908664255
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Ignjatović, Nenad L. AU - Ninkov, Petar AU - Sabetrasekh, Roya AU - Lyngstadaas, Staale Petter AU - Uskoković, Dragan PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7504 AB - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes. T2 - Bio-Medical Materials and Engineering T1 - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide SP - 1647 EP - 1658 DO - 10.3233/BME-140978 ER -
@article{ author = "Ignjatović, Nenad L. and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan", year = "2014", abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.", journal = "Bio-Medical Materials and Engineering", title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide", pages = "1647-1658", doi = "10.3233/BME-140978" }
Ignjatović, N. L., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering, 1647-1658. https://doi.org/10.3233/BME-140978
Ignjatović NL, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;:1647-1658. doi:10.3233/BME-140978 .
Ignjatović, Nenad L., Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering (2014):1647-1658, https://doi.org/10.3233/BME-140978 . .