The role of glucocorticoid receptor phosphorylation in the model of negative affective states
Само за регистроване кориснике
2015
Аутори
Jovičić, MilicaMarić, Nađa P.
Soldatovic, Ivan
Lukić, Iva
Andrić, Sanja
Mihaljević, Marina
Pavlović, Zorana
Mitić, Miloš
Adžić, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objectives. To develop a structural equation model of negative affectivity (NA) that involves interaction of glucocorticoid receptor (GR) signaling, personality dimensions and recent stressful life events. Methods. Seventy participants -35 diagnosed with major depression and 35 healthy controls, were enrolled in the study. Morning plasma cortisol levels were determined by chemiluminescent immunometric assays. Molecular parameters (total nuclear and cytoplasmatic GR, nuclear GR phosphorylated at serine 211 (pGR-211) and at serine 226 (pGR-226) and cytoplasmic FKBP51) were analysed from peripheral blood lymphocytes by Western blot. NA, personality dimensions and stressful life events were assessed by self-report instruments. Results. GR signalling parameters had direct independent effect on measures of NA, with pGR-226 levels showing the strongest correlation, followed by FKBP51 and pGR-211 levels. Neuroticism and extraversion also demonstrated strong independent effect on NA, while rece...nt stressful events did not predict NA directly, but demonstrated a significant effect on personality dimensions. Cortisol, total nuclear GR and total cytoplasmatic GR levels were excluded from the model due to non-significant correlations with NA. Conclusions. Negative affectivity is a transdiagnostic factor in vulnerability to affective disorders and possible therapeutic target. Molecular signature of negative affectivity should incorporate GR phosphorylation with other known biological underpinnings.
Кључне речи:
depression / stress / neuroticism / HPA / modellingИзвор:
World Journal of Biological Psychiatry, 2015, 16, 5, 301-311Финансирање / пројекти:
- Дефинисање кластера молекулских биомаркера за побољшану дијагностику и терапију поремећаја расположења (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.3109/15622975.2014.1000375
ISSN: 1562-2975; 1814-1412
PubMed: 25747256
WoS: 000359980800004
Scopus: 2-s2.0-84938229909
Институција/група
VinčaTY - JOUR AU - Jovičić, Milica AU - Marić, Nađa P. AU - Soldatovic, Ivan AU - Lukić, Iva AU - Andrić, Sanja AU - Mihaljević, Marina AU - Pavlović, Zorana AU - Mitić, Miloš AU - Adžić, Miroslav PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/707 AB - Objectives. To develop a structural equation model of negative affectivity (NA) that involves interaction of glucocorticoid receptor (GR) signaling, personality dimensions and recent stressful life events. Methods. Seventy participants -35 diagnosed with major depression and 35 healthy controls, were enrolled in the study. Morning plasma cortisol levels were determined by chemiluminescent immunometric assays. Molecular parameters (total nuclear and cytoplasmatic GR, nuclear GR phosphorylated at serine 211 (pGR-211) and at serine 226 (pGR-226) and cytoplasmic FKBP51) were analysed from peripheral blood lymphocytes by Western blot. NA, personality dimensions and stressful life events were assessed by self-report instruments. Results. GR signalling parameters had direct independent effect on measures of NA, with pGR-226 levels showing the strongest correlation, followed by FKBP51 and pGR-211 levels. Neuroticism and extraversion also demonstrated strong independent effect on NA, while recent stressful events did not predict NA directly, but demonstrated a significant effect on personality dimensions. Cortisol, total nuclear GR and total cytoplasmatic GR levels were excluded from the model due to non-significant correlations with NA. Conclusions. Negative affectivity is a transdiagnostic factor in vulnerability to affective disorders and possible therapeutic target. Molecular signature of negative affectivity should incorporate GR phosphorylation with other known biological underpinnings. T2 - World Journal of Biological Psychiatry T1 - The role of glucocorticoid receptor phosphorylation in the model of negative affective states VL - 16 IS - 5 SP - 301 EP - 311 DO - 10.3109/15622975.2014.1000375 ER -
@article{ author = "Jovičić, Milica and Marić, Nađa P. and Soldatovic, Ivan and Lukić, Iva and Andrić, Sanja and Mihaljević, Marina and Pavlović, Zorana and Mitić, Miloš and Adžić, Miroslav", year = "2015", abstract = "Objectives. To develop a structural equation model of negative affectivity (NA) that involves interaction of glucocorticoid receptor (GR) signaling, personality dimensions and recent stressful life events. Methods. Seventy participants -35 diagnosed with major depression and 35 healthy controls, were enrolled in the study. Morning plasma cortisol levels were determined by chemiluminescent immunometric assays. Molecular parameters (total nuclear and cytoplasmatic GR, nuclear GR phosphorylated at serine 211 (pGR-211) and at serine 226 (pGR-226) and cytoplasmic FKBP51) were analysed from peripheral blood lymphocytes by Western blot. NA, personality dimensions and stressful life events were assessed by self-report instruments. Results. GR signalling parameters had direct independent effect on measures of NA, with pGR-226 levels showing the strongest correlation, followed by FKBP51 and pGR-211 levels. Neuroticism and extraversion also demonstrated strong independent effect on NA, while recent stressful events did not predict NA directly, but demonstrated a significant effect on personality dimensions. Cortisol, total nuclear GR and total cytoplasmatic GR levels were excluded from the model due to non-significant correlations with NA. Conclusions. Negative affectivity is a transdiagnostic factor in vulnerability to affective disorders and possible therapeutic target. Molecular signature of negative affectivity should incorporate GR phosphorylation with other known biological underpinnings.", journal = "World Journal of Biological Psychiatry", title = "The role of glucocorticoid receptor phosphorylation in the model of negative affective states", volume = "16", number = "5", pages = "301-311", doi = "10.3109/15622975.2014.1000375" }
Jovičić, M., Marić, N. P., Soldatovic, I., Lukić, I., Andrić, S., Mihaljević, M., Pavlović, Z., Mitić, M.,& Adžić, M.. (2015). The role of glucocorticoid receptor phosphorylation in the model of negative affective states. in World Journal of Biological Psychiatry, 16(5), 301-311. https://doi.org/10.3109/15622975.2014.1000375
Jovičić M, Marić NP, Soldatovic I, Lukić I, Andrić S, Mihaljević M, Pavlović Z, Mitić M, Adžić M. The role of glucocorticoid receptor phosphorylation in the model of negative affective states. in World Journal of Biological Psychiatry. 2015;16(5):301-311. doi:10.3109/15622975.2014.1000375 .
Jovičić, Milica, Marić, Nađa P., Soldatovic, Ivan, Lukić, Iva, Andrić, Sanja, Mihaljević, Marina, Pavlović, Zorana, Mitić, Miloš, Adžić, Miroslav, "The role of glucocorticoid receptor phosphorylation in the model of negative affective states" in World Journal of Biological Psychiatry, 16, no. 5 (2015):301-311, https://doi.org/10.3109/15622975.2014.1000375 . .