Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity
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Аутори
Milošević, MajaPetrovic, S.
Stanojević, Ivana
Drakulić, Dunja R.
Velickovic, N.
Horvat, Anica
Чланак у часопису
Метаподаци
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The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex.
Извор:
Russian Journal of Physical Chemistry A, 2009, 83, 9, 1592-1595Напомена:
- 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Sep 24-26, 2008, Belgrade, Serbia
DOI: 10.1134/S0036024409090313
ISSN: 0036-0244
WoS: 000269314100031
Scopus: 2-s2.0-69249229855
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Институција/група
VinčaTY - JOUR AU - Milošević, Maja AU - Petrovic, S. AU - Stanojević, Ivana AU - Drakulić, Dunja R. AU - Velickovic, N. AU - Horvat, Anica PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6813 AB - The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex. T2 - Russian Journal of Physical Chemistry A T1 - Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity VL - 83 IS - 9 SP - 1592 EP - 1595 DO - 10.1134/S0036024409090313 ER -
@article{ author = "Milošević, Maja and Petrovic, S. and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, N. and Horvat, Anica", year = "2009", abstract = "The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex.", journal = "Russian Journal of Physical Chemistry A", title = "Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity", volume = "83", number = "9", pages = "1592-1595", doi = "10.1134/S0036024409090313" }
Milošević, M., Petrovic, S., Stanojević, I., Drakulić, D. R., Velickovic, N.,& Horvat, A.. (2009). Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity. in Russian Journal of Physical Chemistry A, 83(9), 1592-1595. https://doi.org/10.1134/S0036024409090313
Milošević M, Petrovic S, Stanojević I, Drakulić DR, Velickovic N, Horvat A. Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity. in Russian Journal of Physical Chemistry A. 2009;83(9):1592-1595. doi:10.1134/S0036024409090313 .
Milošević, Maja, Petrovic, S., Stanojević, Ivana, Drakulić, Dunja R., Velickovic, N., Horvat, Anica, "Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity" in Russian Journal of Physical Chemistry A, 83, no. 9 (2009):1592-1595, https://doi.org/10.1134/S0036024409090313 . .