Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries
Apstrakt
The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.
Ključne reči:
Mg2+-ATPase / ecto-ATPase / cadmium / mercury / ovary / uterus / ratIzvor:
Annals of the New York Academy of Sciences, 2005, 1048, 445-448Napomena:
- 22nd International Biophysics Symposium, Oct 09-14, 2004, Belgrade, Serbia
DOI: 10.1196/annals.1342.061
ISSN: 0077-8923
PubMed: 16154973
WoS: 000233712300057
Scopus: 2-s2.0-25144472107
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Milošević, Maja AU - Petrovic, S AU - Demajo, Miroslav AU - Horvat, Anica PY - 2005 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6556 AB - The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility. T2 - Annals of the New York Academy of Sciences T1 - Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries VL - 1048 SP - 445 EP - 448 DO - 10.1196/annals.1342.061 ER -
@article{ author = "Milošević, Maja and Petrovic, S and Demajo, Miroslav and Horvat, Anica", year = "2005", abstract = "The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.", journal = "Annals of the New York Academy of Sciences", title = "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries", volume = "1048", pages = "445-448", doi = "10.1196/annals.1342.061" }
Milošević, M., Petrovic, S., Demajo, M.,& Horvat, A.. (2005). Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences, 1048, 445-448. https://doi.org/10.1196/annals.1342.061
Milošević M, Petrovic S, Demajo M, Horvat A. Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences. 2005;1048:445-448. doi:10.1196/annals.1342.061 .
Milošević, Maja, Petrovic, S, Demajo, Miroslav, Horvat, Anica, "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries" in Annals of the New York Academy of Sciences, 1048 (2005):445-448, https://doi.org/10.1196/annals.1342.061 . .