Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP
Samo za registrovane korisnike
2004
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER).
The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive
breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The... findings of this study indicated that (1) sensitivity to radiation and the
proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage.
The experimental study using plasmid DNA has been
performed in order to check how presence of estradiol
modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.
Izvor:
Radiotherapy and Oncology, 2004, 73, S363-S363Napomena:
- 23rd Annual Meeting of the European-Society-for-Therapeutic-Radiology-and-Oncology (ESTRO 23), Oct 24-28, 2004, Amsterdam, Netherlands
- 851 poster
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Institucija/grupa
VinčaTY - CONF AU - Vučić, Vesna AU - Radojčić, Marija AU - Ruždijić, Sabera PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6488 AB - Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER). The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage. The experimental study using plasmid DNA has been performed in order to check how presence of estradiol modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines. C3 - Radiotherapy and Oncology T1 - Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP VL - 73 SP - S363 EP - S363 DO - 10.1016/S0167-8140(04)82718-X UR - https://hdl.handle.net/21.15107/rcub_vinar_6488 ER -
@conference{ author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera", year = "2004", abstract = "Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER). The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage. The experimental study using plasmid DNA has been performed in order to check how presence of estradiol modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.", journal = "Radiotherapy and Oncology", title = "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP", volume = "73", pages = "S363-S363", doi = "10.1016/S0167-8140(04)82718-X", url = "https://hdl.handle.net/21.15107/rcub_vinar_6488" }
Vučić, V., Radojčić, M.,& Ruždijić, S.. (2004). Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology, 73, S363-S363. https://doi.org/10.1016/S0167-8140(04)82718-X https://hdl.handle.net/21.15107/rcub_vinar_6488
Vučić V, Radojčić M, Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology. 2004;73:S363-S363. doi:10.1016/S0167-8140(04)82718-X https://hdl.handle.net/21.15107/rcub_vinar_6488 .
Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP" in Radiotherapy and Oncology, 73 (2004):S363-S363, https://doi.org/10.1016/S0167-8140(04)82718-X ., https://hdl.handle.net/21.15107/rcub_vinar_6488 .