Inhibition of human melanoma cell growth by proton irradiation
Nema prikaza
Autori
Ristić-Fira, AleksandraTodorović, Danijela V.
Petrović, Ivan M.
Ruzvdijic, S
Raffaele, L
Sabini, MG
Cirrone, Giuseppe Antonio Pablo
Cuttone, Giacomo
Farruggia, G
Masotti, L
Kanazir, Dušan T.
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
Ključne reči:
HTB63 human melanoma cells / proton irradiation / cell cycle / apoptosisIzvor:
Physica Medica, 2001, 17, 71-75Napomena:
- International Conference on Ocular Pathologies Therapy with Proton Beams, Oct 12-13, 2000, Catania, Italy
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Ristić-Fira, Aleksandra AU - Todorović, Danijela V. AU - Petrović, Ivan M. AU - Ruzvdijic, S AU - Raffaele, L AU - Sabini, MG AU - Cirrone, Giuseppe Antonio Pablo AU - Cuttone, Giacomo AU - Farruggia, G AU - Masotti, L AU - Kanazir, Dušan T. PY - 2001 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6321 AB - The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis. T2 - Physica Medica T1 - Inhibition of human melanoma cell growth by proton irradiation VL - 17 SP - 71 EP - 75 UR - https://hdl.handle.net/21.15107/rcub_vinar_6321 ER -
@article{ author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Petrović, Ivan M. and Ruzvdijic, S and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.", year = "2001", abstract = "The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.", journal = "Physica Medica", title = "Inhibition of human melanoma cell growth by proton irradiation", volume = "17", pages = "71-75", url = "https://hdl.handle.net/21.15107/rcub_vinar_6321" }
Ristić-Fira, A., Todorović, D. V., Petrović, I. M., Ruzvdijic, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica, 17, 71-75. https://hdl.handle.net/21.15107/rcub_vinar_6321
Ristić-Fira A, Todorović DV, Petrović IM, Ruzvdijic S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica. 2001;17:71-75. https://hdl.handle.net/21.15107/rcub_vinar_6321 .
Ristić-Fira, Aleksandra, Todorović, Danijela V., Petrović, Ivan M., Ruzvdijic, S, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "Inhibition of human melanoma cell growth by proton irradiation" in Physica Medica, 17 (2001):71-75, https://hdl.handle.net/21.15107/rcub_vinar_6321 .