A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example
Abstract
In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region.
Keywords:
HCV genotyping / RT-PCR / HCV subtypeSource:
Clinical Chemistry and Laboratory Medicine, 1998, 36, 8, 587-588Note:
- 1st IFCC Roche Conference on Recent Progress in Molecular biology Technology, Mar 15-18, 1998, Singapore, Singapore
DOI: 10.1515/CCLM.1998.101
ISSN: 1434-6621
PubMed: 9806465
WoS: 000076742100018
Scopus: 2-s2.0-0031671823
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VinčaTY - JOUR AU - Alavantić, Dragan AU - Glišić, Sanja AU - Radovanovic, N AU - Romic, M PY - 1998 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6259 AB - In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region. T2 - Clinical Chemistry and Laboratory Medicine T1 - A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example VL - 36 IS - 8 SP - 587 EP - 588 DO - 10.1515/CCLM.1998.101 ER -
@article{ author = "Alavantić, Dragan and Glišić, Sanja and Radovanovic, N and Romic, M", year = "1998", abstract = "In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region.", journal = "Clinical Chemistry and Laboratory Medicine", title = "A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example", volume = "36", number = "8", pages = "587-588", doi = "10.1515/CCLM.1998.101" }
Alavantić, D., Glišić, S., Radovanovic, N.,& Romic, M.. (1998). A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example. in Clinical Chemistry and Laboratory Medicine, 36(8), 587-588. https://doi.org/10.1515/CCLM.1998.101
Alavantić D, Glišić S, Radovanovic N, Romic M. A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example. in Clinical Chemistry and Laboratory Medicine. 1998;36(8):587-588. doi:10.1515/CCLM.1998.101 .
Alavantić, Dragan, Glišić, Sanja, Radovanovic, N, Romic, M, "A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example" in Clinical Chemistry and Laboratory Medicine, 36, no. 8 (1998):587-588, https://doi.org/10.1515/CCLM.1998.101 . .