The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings
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2014
Authors
Stojković, Ljiljana S.Stanković, Aleksandra
Đurić, Tamara
Dinčić, Evica
Alavantić, Dragan
Živković, Maja
Article (Published version)
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CXC ligand 16 (CXCL16) is a multifunctional chemokine involved in cell adhesion and chemoattraction as well as in the scavenging of oxidized lipoproteins. Experimental data suggest the roles of CXCL16 in pathogenesis of multiple sclerosis (MS). A181V polymorphism in the human CXCL16 gene has been associated with the clinical course of certain chronic inflammatory diseases. The aim of this study was to analyze the effects of CXCL16 A181V polymorphism on: (1) susceptibility to MS and disease course, (2) peripheral blood mononuclear cells (PBMC) CXCL16 mRNA levels and plasma soluble CXCL16 levels of patients with MS and healthy controls. In this study, 459 MS patients and 303 controls were included. Real-time PCR-based methods were applied for genotyping of CXCL16 A181V and for CXCL16 gene expression analysis. Quantitative sandwich enzyme immunoassay was performed for quantification of plasma soluble CXCL16. CXCL16 AA genotype had a significant protective effect on MS susceptibility in wo...men (OR = 0.53, +/- 95 % CI = 0.35-0.82, p = 0.004). The V allele-containing genotypes were associated with significantly higher CXCL16 mRNA levels in PBMC of both female (mean factor = 1.81, S.E. = 1.14-2.77, p LT 0.01) and male (mean factor = 1.58, S.E. = 1.35-1.73, p LT 0.01) controls. No significant association of the CXCL16 polymorphism was established either with soluble CXCL16 plasma levels or with clinical parameters and course of MS. The main finding of this study is gender-specific association of CXCL16 A181V polymorphism with susceptibility to MS in females. The current results should be replicated and validated in the larger sample group.
Keywords:
CXCL16 / Gene / Polymorphism / A181V / Multiple sclerosisSource:
Journal of Neurology, 2014, 261, 8, 1544-1551Funding / projects:
- Genetic basis of human vascular and inflammatory diseases (RS-MESTD-Basic Research (BR or ON)-175085)
- An integral study to identify the regional genetic and environmental risk factors for the common noncommunicable diseases in the human population of Serbia - INGEMA_S (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41028)
DOI: 10.1007/s00415-014-7379-7
ISSN: 0340-5354; 1432-1459
PubMed: 24854635
WoS: 000340052000013
Scopus: 2-s2.0-84906093770
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VinčaTY - JOUR AU - Stojković, Ljiljana S. AU - Stanković, Aleksandra AU - Đurić, Tamara AU - Dinčić, Evica AU - Alavantić, Dragan AU - Živković, Maja PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/61 AB - CXC ligand 16 (CXCL16) is a multifunctional chemokine involved in cell adhesion and chemoattraction as well as in the scavenging of oxidized lipoproteins. Experimental data suggest the roles of CXCL16 in pathogenesis of multiple sclerosis (MS). A181V polymorphism in the human CXCL16 gene has been associated with the clinical course of certain chronic inflammatory diseases. The aim of this study was to analyze the effects of CXCL16 A181V polymorphism on: (1) susceptibility to MS and disease course, (2) peripheral blood mononuclear cells (PBMC) CXCL16 mRNA levels and plasma soluble CXCL16 levels of patients with MS and healthy controls. In this study, 459 MS patients and 303 controls were included. Real-time PCR-based methods were applied for genotyping of CXCL16 A181V and for CXCL16 gene expression analysis. Quantitative sandwich enzyme immunoassay was performed for quantification of plasma soluble CXCL16. CXCL16 AA genotype had a significant protective effect on MS susceptibility in women (OR = 0.53, +/- 95 % CI = 0.35-0.82, p = 0.004). The V allele-containing genotypes were associated with significantly higher CXCL16 mRNA levels in PBMC of both female (mean factor = 1.81, S.E. = 1.14-2.77, p LT 0.01) and male (mean factor = 1.58, S.E. = 1.35-1.73, p LT 0.01) controls. No significant association of the CXCL16 polymorphism was established either with soluble CXCL16 plasma levels or with clinical parameters and course of MS. The main finding of this study is gender-specific association of CXCL16 A181V polymorphism with susceptibility to MS in females. The current results should be replicated and validated in the larger sample group. T2 - Journal of Neurology T1 - The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings VL - 261 IS - 8 SP - 1544 EP - 1551 DO - 10.1007/s00415-014-7379-7 ER -
@article{ author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Đurić, Tamara and Dinčić, Evica and Alavantić, Dragan and Živković, Maja", year = "2014", abstract = "CXC ligand 16 (CXCL16) is a multifunctional chemokine involved in cell adhesion and chemoattraction as well as in the scavenging of oxidized lipoproteins. Experimental data suggest the roles of CXCL16 in pathogenesis of multiple sclerosis (MS). A181V polymorphism in the human CXCL16 gene has been associated with the clinical course of certain chronic inflammatory diseases. The aim of this study was to analyze the effects of CXCL16 A181V polymorphism on: (1) susceptibility to MS and disease course, (2) peripheral blood mononuclear cells (PBMC) CXCL16 mRNA levels and plasma soluble CXCL16 levels of patients with MS and healthy controls. In this study, 459 MS patients and 303 controls were included. Real-time PCR-based methods were applied for genotyping of CXCL16 A181V and for CXCL16 gene expression analysis. Quantitative sandwich enzyme immunoassay was performed for quantification of plasma soluble CXCL16. CXCL16 AA genotype had a significant protective effect on MS susceptibility in women (OR = 0.53, +/- 95 % CI = 0.35-0.82, p = 0.004). The V allele-containing genotypes were associated with significantly higher CXCL16 mRNA levels in PBMC of both female (mean factor = 1.81, S.E. = 1.14-2.77, p LT 0.01) and male (mean factor = 1.58, S.E. = 1.35-1.73, p LT 0.01) controls. No significant association of the CXCL16 polymorphism was established either with soluble CXCL16 plasma levels or with clinical parameters and course of MS. The main finding of this study is gender-specific association of CXCL16 A181V polymorphism with susceptibility to MS in females. The current results should be replicated and validated in the larger sample group.", journal = "Journal of Neurology", title = "The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings", volume = "261", number = "8", pages = "1544-1551", doi = "10.1007/s00415-014-7379-7" }
Stojković, L. S., Stanković, A., Đurić, T., Dinčić, E., Alavantić, D.,& Živković, M.. (2014). The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings. in Journal of Neurology, 261(8), 1544-1551. https://doi.org/10.1007/s00415-014-7379-7
Stojković LS, Stanković A, Đurić T, Dinčić E, Alavantić D, Živković M. The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings. in Journal of Neurology. 2014;261(8):1544-1551. doi:10.1007/s00415-014-7379-7 .
Stojković, Ljiljana S., Stanković, Aleksandra, Đurić, Tamara, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings" in Journal of Neurology, 261, no. 8 (2014):1544-1551, https://doi.org/10.1007/s00415-014-7379-7 . .