Photodynamic antibacterial effect of graphene quantum dots
No Thumbnail
Authors
Ristić, Biljana Z.Milenković, Marina
Dakic, Ivana R.
Todorović-Marković, Biljana
Milosavljević, Momir
Budimir, Milica
Paunović, Verica G.
Dramićanin, Miroslav
Marković, Zoran M.
Trajković, Vladimir S.
Article
Metadata
Show full item recordAbstract
Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells we...re markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords:
Graphene / Quantum dot / Photodynamic / Antibacterial / Oxidative stressSource:
Biomaterials, 2014, 35, 15, 4428-4435Funding / projects:
- Modulation of intracellular energy balance-controlling signalling pathways in therapy of cancer and neuro-immuno-endocrine disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41025)
- Thin films of single wall carbon nanotubes and graphene for electronic application (RS-MESTD-Basic Research (BR or ON)-172003)
DOI: 10.1016/j.biomaterials.2014.02.014
ISSN: 0142-9612; 1878-5905
PubMed: 24612819
WoS: 000334819800002
Scopus: 2-s2.0-84896056867
Collections
Institution/Community
VinčaTY - JOUR AU - Ristić, Biljana Z. AU - Milenković, Marina AU - Dakic, Ivana R. AU - Todorović-Marković, Biljana AU - Milosavljević, Momir AU - Budimir, Milica AU - Paunović, Verica G. AU - Dramićanin, Miroslav AU - Marković, Zoran M. AU - Trajković, Vladimir S. PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5964 AB - Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells were markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD. (C) 2014 Elsevier Ltd. All rights reserved. T2 - Biomaterials T1 - Photodynamic antibacterial effect of graphene quantum dots VL - 35 IS - 15 SP - 4428 EP - 4435 DO - 10.1016/j.biomaterials.2014.02.014 ER -
@article{ author = "Ristić, Biljana Z. and Milenković, Marina and Dakic, Ivana R. and Todorović-Marković, Biljana and Milosavljević, Momir and Budimir, Milica and Paunović, Verica G. and Dramićanin, Miroslav and Marković, Zoran M. and Trajković, Vladimir S.", year = "2014", abstract = "Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells were markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD. (C) 2014 Elsevier Ltd. All rights reserved.", journal = "Biomaterials", title = "Photodynamic antibacterial effect of graphene quantum dots", volume = "35", number = "15", pages = "4428-4435", doi = "10.1016/j.biomaterials.2014.02.014" }
Ristić, B. Z., Milenković, M., Dakic, I. R., Todorović-Marković, B., Milosavljević, M., Budimir, M., Paunović, V. G., Dramićanin, M., Marković, Z. M.,& Trajković, V. S.. (2014). Photodynamic antibacterial effect of graphene quantum dots. in Biomaterials, 35(15), 4428-4435. https://doi.org/10.1016/j.biomaterials.2014.02.014
Ristić BZ, Milenković M, Dakic IR, Todorović-Marković B, Milosavljević M, Budimir M, Paunović VG, Dramićanin M, Marković ZM, Trajković VS. Photodynamic antibacterial effect of graphene quantum dots. in Biomaterials. 2014;35(15):4428-4435. doi:10.1016/j.biomaterials.2014.02.014 .
Ristić, Biljana Z., Milenković, Marina, Dakic, Ivana R., Todorović-Marković, Biljana, Milosavljević, Momir, Budimir, Milica, Paunović, Verica G., Dramićanin, Miroslav, Marković, Zoran M., Trajković, Vladimir S., "Photodynamic antibacterial effect of graphene quantum dots" in Biomaterials, 35, no. 15 (2014):4428-4435, https://doi.org/10.1016/j.biomaterials.2014.02.014 . .