Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2
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Autori
Jovanović, PredragSpasojević, Nataša
Stefanović, Bojana
Božović, N.
Jasnic, N.
Đorđević, Jelena D.
Dronjak, Slađana
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not stat...istically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.
Ključne reči:
Oxytocin / Adrenal-medulla / Epinephrine / Norepinephrine / Vesicular monoamine transporter 2Izvor:
Peptides, 2014, 51, 110-114Finansiranje / projekti:
- Molekularni mehanizmi patofizioloških promena u ćelijama centralnog nervnog sistema i perifernog tkiva kod sisara (RS-MESTD-Basic Research (BR or ON)-173044)
- Efekat metaboličkih i nemetaboličkih stresora na ekspresiju i delovanje neuroendokrinih regulatora energetske homeostaze (RS-MESTD-Basic Research (BR or ON)-173023)
DOI: 10.1016/j.peptides.2013.11.001
ISSN: 0196-9781; 1873-5169
PubMed: 24239562
WoS: 000329208100017
Scopus: 2-s2.0-84889667266
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Jovanović, Predrag AU - Spasojević, Nataša AU - Stefanović, Bojana AU - Božović, N. AU - Jasnic, N. AU - Đorđević, Jelena D. AU - Dronjak, Slađana PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5825 AB - The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved. T2 - Peptides T1 - Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2 VL - 51 SP - 110 EP - 114 DO - 10.1016/j.peptides.2013.11.001 ER -
@article{ author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Božović, N. and Jasnic, N. and Đorđević, Jelena D. and Dronjak, Slađana", year = "2014", abstract = "The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.", journal = "Peptides", title = "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2", volume = "51", pages = "110-114", doi = "10.1016/j.peptides.2013.11.001" }
Jovanović, P., Spasojević, N., Stefanović, B., Božović, N., Jasnic, N., Đorđević, J. D.,& Dronjak, S.. (2014). Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides, 51, 110-114. https://doi.org/10.1016/j.peptides.2013.11.001
Jovanović P, Spasojević N, Stefanović B, Božović N, Jasnic N, Đorđević JD, Dronjak S. Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides. 2014;51:110-114. doi:10.1016/j.peptides.2013.11.001 .
Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Božović, N., Jasnic, N., Đorđević, Jelena D., Dronjak, Slađana, "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2" in Peptides, 51 (2014):110-114, https://doi.org/10.1016/j.peptides.2013.11.001 . .