Altered LINE-1 Methylation in Mothers of Children with Down Syndrome
Autori
Babić Božović, IvanaStanković, Aleksandra
Živković, Maja
Vraneković, Jadranka
Kapović, Miljenko
Brajenović-Milić, Bojana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Down syndrome (DS, also known as trisomy 21) most often results from chromosomal nondisjunction during oogenesis. Numerous studies sustain a causal link between global DNA hypomethylation and genetic instability. It has been suggested that DNA hypomethylation might affect the structure and dynamics of chromatin regions that are critical for chromosome stability and segregation, thus favouring chromosomal nondisjunction during meiosis. Maternal global DNA hypomethylation has not yet been analyzed as a potential risk factor for chromosome 21 nondisjunction. This study aimed to asses the risk for DS in association with maternal global DNA methylation and the impact of endogenous and exogenous factors that reportedly influence DNA methylation status. Global DNA methylation was analyzed in peripheral blood lymphocytes by quantifying LINE-1 methylation using the MethyLight method. Levels of global DNA methylation were significantly lower among mothers of children with maternally derived tris...omy 21 than among control mothers (P = 0.000). The combination of MTHFR C677T genotype and diet significantly influenced global DNA methylation (R-2 = 4.5%, P = 0.046). The lowest values of global DNA methylation were observed in mothers with MTHFR 677 CT+TT genotype and low dietary folate. Although our findings revealed an association between maternal global DNA hypomethylation and trisomy 21 of maternal origin, further progress and final conclusions regarding the role of global DNA methylation and the occurrence of trisomy 21 are facing major challenges.
Izvor:
PLOS One, 2015, 10, 5Finansiranje / projekti:
- University of Rijeka, Rijeka, Croatia [13.06.1.2.38]
DOI: 10.1371/journal.pone.0127423
ISSN: 1932-6203
WoS: 000355185600082
Scopus: 2-s2.0-84957549144
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Babić Božović, Ivana AU - Stanković, Aleksandra AU - Živković, Maja AU - Vraneković, Jadranka AU - Kapović, Miljenko AU - Brajenović-Milić, Bojana PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/564 AB - Down syndrome (DS, also known as trisomy 21) most often results from chromosomal nondisjunction during oogenesis. Numerous studies sustain a causal link between global DNA hypomethylation and genetic instability. It has been suggested that DNA hypomethylation might affect the structure and dynamics of chromatin regions that are critical for chromosome stability and segregation, thus favouring chromosomal nondisjunction during meiosis. Maternal global DNA hypomethylation has not yet been analyzed as a potential risk factor for chromosome 21 nondisjunction. This study aimed to asses the risk for DS in association with maternal global DNA methylation and the impact of endogenous and exogenous factors that reportedly influence DNA methylation status. Global DNA methylation was analyzed in peripheral blood lymphocytes by quantifying LINE-1 methylation using the MethyLight method. Levels of global DNA methylation were significantly lower among mothers of children with maternally derived trisomy 21 than among control mothers (P = 0.000). The combination of MTHFR C677T genotype and diet significantly influenced global DNA methylation (R-2 = 4.5%, P = 0.046). The lowest values of global DNA methylation were observed in mothers with MTHFR 677 CT+TT genotype and low dietary folate. Although our findings revealed an association between maternal global DNA hypomethylation and trisomy 21 of maternal origin, further progress and final conclusions regarding the role of global DNA methylation and the occurrence of trisomy 21 are facing major challenges. T2 - PLOS One T1 - Altered LINE-1 Methylation in Mothers of Children with Down Syndrome VL - 10 IS - 5 DO - 10.1371/journal.pone.0127423 ER -
@article{ author = "Babić Božović, Ivana and Stanković, Aleksandra and Živković, Maja and Vraneković, Jadranka and Kapović, Miljenko and Brajenović-Milić, Bojana", year = "2015", abstract = "Down syndrome (DS, also known as trisomy 21) most often results from chromosomal nondisjunction during oogenesis. Numerous studies sustain a causal link between global DNA hypomethylation and genetic instability. It has been suggested that DNA hypomethylation might affect the structure and dynamics of chromatin regions that are critical for chromosome stability and segregation, thus favouring chromosomal nondisjunction during meiosis. Maternal global DNA hypomethylation has not yet been analyzed as a potential risk factor for chromosome 21 nondisjunction. This study aimed to asses the risk for DS in association with maternal global DNA methylation and the impact of endogenous and exogenous factors that reportedly influence DNA methylation status. Global DNA methylation was analyzed in peripheral blood lymphocytes by quantifying LINE-1 methylation using the MethyLight method. Levels of global DNA methylation were significantly lower among mothers of children with maternally derived trisomy 21 than among control mothers (P = 0.000). The combination of MTHFR C677T genotype and diet significantly influenced global DNA methylation (R-2 = 4.5%, P = 0.046). The lowest values of global DNA methylation were observed in mothers with MTHFR 677 CT+TT genotype and low dietary folate. Although our findings revealed an association between maternal global DNA hypomethylation and trisomy 21 of maternal origin, further progress and final conclusions regarding the role of global DNA methylation and the occurrence of trisomy 21 are facing major challenges.", journal = "PLOS One", title = "Altered LINE-1 Methylation in Mothers of Children with Down Syndrome", volume = "10", number = "5", doi = "10.1371/journal.pone.0127423" }
Babić Božović, I., Stanković, A., Živković, M., Vraneković, J., Kapović, M.,& Brajenović-Milić, B.. (2015). Altered LINE-1 Methylation in Mothers of Children with Down Syndrome. in PLOS One, 10(5). https://doi.org/10.1371/journal.pone.0127423
Babić Božović I, Stanković A, Živković M, Vraneković J, Kapović M, Brajenović-Milić B. Altered LINE-1 Methylation in Mothers of Children with Down Syndrome. in PLOS One. 2015;10(5). doi:10.1371/journal.pone.0127423 .
Babić Božović, Ivana, Stanković, Aleksandra, Živković, Maja, Vraneković, Jadranka, Kapović, Miljenko, Brajenović-Milić, Bojana, "Altered LINE-1 Methylation in Mothers of Children with Down Syndrome" in PLOS One, 10, no. 5 (2015), https://doi.org/10.1371/journal.pone.0127423 . .