In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility
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Čairović, AleksandraDjordjevic, Ivan
Bulatovic, Mirna
Mojic, Marija
Momčilović, Miljana
Stošić-Grujičić, Stanislava
Maksimović, Vesna
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Stamenkovic, Dragoslav
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The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy ...promoted its toxicity.
Keywords:
Cobalt-chromium (Co-Cr) alloys / Nickel-chromium (Ni-Cr) alloys / Recasting / apoptosis / ROS / RNSSource:
Digest Journal of Nanomaterials and Biostructures, 2013, 8, 2, 877-U461Funding / projects:
- Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine (RS-45012)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-173013)
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VinčaTY - JOUR AU - Čairović, Aleksandra AU - Djordjevic, Ivan AU - Bulatovic, Mirna AU - Mojic, Marija AU - Momčilović, Miljana AU - Stošić-Grujičić, Stanislava AU - Maksimović, Vesna AU - Maksimović-Ivanić, Danijela AU - Mijatović, Sanja AU - Stamenkovic, Dragoslav PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5620 AB - The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity. T2 - Digest Journal of Nanomaterials and Biostructures T1 - In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility VL - 8 IS - 2 SP - 877 EP - U461 UR - https://hdl.handle.net/21.15107/rcub_vinar_5620 ER -
@article{ author = "Čairović, Aleksandra and Djordjevic, Ivan and Bulatovic, Mirna and Mojic, Marija and Momčilović, Miljana and Stošić-Grujičić, Stanislava and Maksimović, Vesna and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Stamenkovic, Dragoslav", year = "2013", abstract = "The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.", journal = "Digest Journal of Nanomaterials and Biostructures", title = "In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility", volume = "8", number = "2", pages = "877-U461", url = "https://hdl.handle.net/21.15107/rcub_vinar_5620" }
Čairović, A., Djordjevic, I., Bulatovic, M., Mojic, M., Momčilović, M., Stošić-Grujičić, S., Maksimović, V., Maksimović-Ivanić, D., Mijatović, S.,& Stamenkovic, D.. (2013). In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility. in Digest Journal of Nanomaterials and Biostructures, 8(2), 877-U461. https://hdl.handle.net/21.15107/rcub_vinar_5620
Čairović A, Djordjevic I, Bulatovic M, Mojic M, Momčilović M, Stošić-Grujičić S, Maksimović V, Maksimović-Ivanić D, Mijatović S, Stamenkovic D. In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility. in Digest Journal of Nanomaterials and Biostructures. 2013;8(2):877-U461. https://hdl.handle.net/21.15107/rcub_vinar_5620 .
Čairović, Aleksandra, Djordjevic, Ivan, Bulatovic, Mirna, Mojic, Marija, Momčilović, Miljana, Stošić-Grujičić, Stanislava, Maksimović, Vesna, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Stamenkovic, Dragoslav, "In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility" in Digest Journal of Nanomaterials and Biostructures, 8, no. 2 (2013):877-U461, https://hdl.handle.net/21.15107/rcub_vinar_5620 .