Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia
Аутори
Petrović, SandraLeskovac, Andreja
Joksić, Ivana
Filipović, Jelena G.
Vujić, Dragana
Guć-Šćekić, Marija
Joksić, Gordana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.
Кључне речи:
Apoptosis / Fanconi anemia / heterozygous carriers / radiosensitivityИзвор:
Current Science, 2013, 105, 1, 56-60Финансирање / пројекти:
- Радиосензитивност хуманог генома (RS-MESTD-Basic Research (BR or ON)-173046)
Колекције
Институција/група
VinčaTY - JOUR AU - Petrović, Sandra AU - Leskovac, Andreja AU - Joksić, Ivana AU - Filipović, Jelena G. AU - Vujić, Dragana AU - Guć-Šćekić, Marija AU - Joksić, Gordana PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5606 AB - Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity. T2 - Current Science T1 - Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia VL - 105 IS - 1 SP - 56 EP - 60 UR - https://hdl.handle.net/21.15107/rcub_vinar_5606 ER -
@article{ author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Vujić, Dragana and Guć-Šćekić, Marija and Joksić, Gordana", year = "2013", abstract = "Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.", journal = "Current Science", title = "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia", volume = "105", number = "1", pages = "56-60", url = "https://hdl.handle.net/21.15107/rcub_vinar_5606" }
Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G., Vujić, D., Guć-Šćekić, M.,& Joksić, G.. (2013). Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science, 105(1), 56-60. https://hdl.handle.net/21.15107/rcub_vinar_5606
Petrović S, Leskovac A, Joksić I, Filipović JG, Vujić D, Guć-Šćekić M, Joksić G. Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science. 2013;105(1):56-60. https://hdl.handle.net/21.15107/rcub_vinar_5606 .
Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Vujić, Dragana, Guć-Šćekić, Marija, Joksić, Gordana, "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia" in Current Science, 105, no. 1 (2013):56-60, https://hdl.handle.net/21.15107/rcub_vinar_5606 .