Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats
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Spasojević, NatašaStanisavljevic, Danijela
Gavrilović, Ljubica
Jovanović, Predrag
Cucakovic, Aleksandar
Dronjak, Slađana
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OBJECTIVES: Right-left asymmetry of human brain function has been known for a century. Brain asymmetry and lateralization has been observed at the neurochemical level. At the neurochemical level, it is important to further correlate changes in monoaminergic activity with the synthesis and reuptake of these monoamines. The aim of the present study was to analyze the effect of social isolation on catecholamine stores as well as on the regulation of catecholamine synthesis and uptake in the right and left hippocampus. METHODS: We examined changes in protein levels of dopamine-beta-hydroxylase (DBH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the right and left hippocampus of socially isolated adult male rats during 12 weeks by Western blot analysis. RESULTS: Chronic isolation stress reduced norepinephrine content in the right hippocampus. No changes were observed in protein levels of DBH and NET in the right hippocampus, whereas expression of this no...repinephrine synthetizing enzyme and transporter were elevated in the left hippocampus. On the other hand, chronic isolation stress caused reduction of VMAT2 protein in the right hippocampus. CONCLUSION: Our results reveale not only the lateralization of stress regulatory system but they also show that long-term isolation stress produces right-left asymmetry of the hippocampus norepinephrine, different regulation of the catecholamines synthesis and reuptake.
Keywords:
hippocampus / chronic stress / right-left asymmetry / catecholamines / enzymes / ratsSource:
Neuroendocrinology Letters, 2012, 33, 6, 631-635Funding / projects:
- Molecular mechanisms of cellular responses on pathological changes in central neuronal system and peripheral organs of mammals (RS-MESTD-Basic Research (BR or ON)-173044)
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VinčaTY - JOUR AU - Spasojević, Nataša AU - Stanisavljevic, Danijela AU - Gavrilović, Ljubica AU - Jovanović, Predrag AU - Cucakovic, Aleksandar AU - Dronjak, Slađana PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5196 AB - OBJECTIVES: Right-left asymmetry of human brain function has been known for a century. Brain asymmetry and lateralization has been observed at the neurochemical level. At the neurochemical level, it is important to further correlate changes in monoaminergic activity with the synthesis and reuptake of these monoamines. The aim of the present study was to analyze the effect of social isolation on catecholamine stores as well as on the regulation of catecholamine synthesis and uptake in the right and left hippocampus. METHODS: We examined changes in protein levels of dopamine-beta-hydroxylase (DBH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the right and left hippocampus of socially isolated adult male rats during 12 weeks by Western blot analysis. RESULTS: Chronic isolation stress reduced norepinephrine content in the right hippocampus. No changes were observed in protein levels of DBH and NET in the right hippocampus, whereas expression of this norepinephrine synthetizing enzyme and transporter were elevated in the left hippocampus. On the other hand, chronic isolation stress caused reduction of VMAT2 protein in the right hippocampus. CONCLUSION: Our results reveale not only the lateralization of stress regulatory system but they also show that long-term isolation stress produces right-left asymmetry of the hippocampus norepinephrine, different regulation of the catecholamines synthesis and reuptake. T2 - Neuroendocrinology Letters T1 - Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats VL - 33 IS - 6 SP - 631 EP - 635 UR - https://hdl.handle.net/21.15107/rcub_vinar_5196 ER -
@article{ author = "Spasojević, Nataša and Stanisavljevic, Danijela and Gavrilović, Ljubica and Jovanović, Predrag and Cucakovic, Aleksandar and Dronjak, Slađana", year = "2012", abstract = "OBJECTIVES: Right-left asymmetry of human brain function has been known for a century. Brain asymmetry and lateralization has been observed at the neurochemical level. At the neurochemical level, it is important to further correlate changes in monoaminergic activity with the synthesis and reuptake of these monoamines. The aim of the present study was to analyze the effect of social isolation on catecholamine stores as well as on the regulation of catecholamine synthesis and uptake in the right and left hippocampus. METHODS: We examined changes in protein levels of dopamine-beta-hydroxylase (DBH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the right and left hippocampus of socially isolated adult male rats during 12 weeks by Western blot analysis. RESULTS: Chronic isolation stress reduced norepinephrine content in the right hippocampus. No changes were observed in protein levels of DBH and NET in the right hippocampus, whereas expression of this norepinephrine synthetizing enzyme and transporter were elevated in the left hippocampus. On the other hand, chronic isolation stress caused reduction of VMAT2 protein in the right hippocampus. CONCLUSION: Our results reveale not only the lateralization of stress regulatory system but they also show that long-term isolation stress produces right-left asymmetry of the hippocampus norepinephrine, different regulation of the catecholamines synthesis and reuptake.", journal = "Neuroendocrinology Letters", title = "Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats", volume = "33", number = "6", pages = "631-635", url = "https://hdl.handle.net/21.15107/rcub_vinar_5196" }
Spasojević, N., Stanisavljevic, D., Gavrilović, L., Jovanović, P., Cucakovic, A.,& Dronjak, S.. (2012). Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats. in Neuroendocrinology Letters, 33(6), 631-635. https://hdl.handle.net/21.15107/rcub_vinar_5196
Spasojević N, Stanisavljevic D, Gavrilović L, Jovanović P, Cucakovic A, Dronjak S. Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats. in Neuroendocrinology Letters. 2012;33(6):631-635. https://hdl.handle.net/21.15107/rcub_vinar_5196 .
Spasojević, Nataša, Stanisavljevic, Danijela, Gavrilović, Ljubica, Jovanović, Predrag, Cucakovic, Aleksandar, Dronjak, Slađana, "Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats" in Neuroendocrinology Letters, 33, no. 6 (2012):631-635, https://hdl.handle.net/21.15107/rcub_vinar_5196 .