Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Depression is associated with increased risk of coronary heart diseases. Selective serotonin reuptake inhibitors (SSRIs) have been proved to be very effective in normalizing symptoms of depression, but the data on possible influence of these drugs on cardiovascular function is controversial. Applying Taqman RT-PCR assay, the effect of chronic treatment with a SSRI antidepressant fluoxetine has been investigated on gene expression of catecholamine biosynthetic enzymes in all four heart chambers of rats with signs of depression. Depression was induced by exposing the animals to chronic unpredictable mild stress (CUMS). Tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) mRNA levels were decreased both in right and left atria, while phenylethanolamine N-methyltransferase (PNMT) mRNAs were increased in left atria and both ventricles of depression model rats. Fluoxetine elevated gene expression of TH and DBH in atria, but did not influence this process in the ventricles. Also, thi...s antidepressant did not express a significant effect on the level of PNMT mRNA both in atria and ventricles. These results indicate that fluoxetine acted stimulating noradrenaline synthesis in the heart, which could lead to increased risk of heart disease.
Ključne reči:
Antidepressant / Catecholamine enzymes / Depression / Gene expression / HeartIzvor:
Indian Journal of Experimental Biology, 2012, 50, 11, 771-775Finansiranje / projekti:
- Molekularni mehanizmi patofizioloških promena u ćelijama centralnog nervnog sistema i perifernog tkiva kod sisara (RS-MESTD-Basic Research (BR or ON)-173044)
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Spasojević, Nataša AU - Jovanović, Predrag AU - Dronjak, Slađana PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5153 AB - Depression is associated with increased risk of coronary heart diseases. Selective serotonin reuptake inhibitors (SSRIs) have been proved to be very effective in normalizing symptoms of depression, but the data on possible influence of these drugs on cardiovascular function is controversial. Applying Taqman RT-PCR assay, the effect of chronic treatment with a SSRI antidepressant fluoxetine has been investigated on gene expression of catecholamine biosynthetic enzymes in all four heart chambers of rats with signs of depression. Depression was induced by exposing the animals to chronic unpredictable mild stress (CUMS). Tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) mRNA levels were decreased both in right and left atria, while phenylethanolamine N-methyltransferase (PNMT) mRNAs were increased in left atria and both ventricles of depression model rats. Fluoxetine elevated gene expression of TH and DBH in atria, but did not influence this process in the ventricles. Also, this antidepressant did not express a significant effect on the level of PNMT mRNA both in atria and ventricles. These results indicate that fluoxetine acted stimulating noradrenaline synthesis in the heart, which could lead to increased risk of heart disease. T2 - Indian Journal of Experimental Biology T1 - Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats VL - 50 IS - 11 SP - 771 EP - 775 UR - https://hdl.handle.net/21.15107/rcub_vinar_5153 ER -
@article{ author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana", year = "2012", abstract = "Depression is associated with increased risk of coronary heart diseases. Selective serotonin reuptake inhibitors (SSRIs) have been proved to be very effective in normalizing symptoms of depression, but the data on possible influence of these drugs on cardiovascular function is controversial. Applying Taqman RT-PCR assay, the effect of chronic treatment with a SSRI antidepressant fluoxetine has been investigated on gene expression of catecholamine biosynthetic enzymes in all four heart chambers of rats with signs of depression. Depression was induced by exposing the animals to chronic unpredictable mild stress (CUMS). Tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) mRNA levels were decreased both in right and left atria, while phenylethanolamine N-methyltransferase (PNMT) mRNAs were increased in left atria and both ventricles of depression model rats. Fluoxetine elevated gene expression of TH and DBH in atria, but did not influence this process in the ventricles. Also, this antidepressant did not express a significant effect on the level of PNMT mRNA both in atria and ventricles. These results indicate that fluoxetine acted stimulating noradrenaline synthesis in the heart, which could lead to increased risk of heart disease.", journal = "Indian Journal of Experimental Biology", title = "Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats", volume = "50", number = "11", pages = "771-775", url = "https://hdl.handle.net/21.15107/rcub_vinar_5153" }
Spasojević, N., Jovanović, P.,& Dronjak, S.. (2012). Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats. in Indian Journal of Experimental Biology, 50(11), 771-775. https://hdl.handle.net/21.15107/rcub_vinar_5153
Spasojević N, Jovanović P, Dronjak S. Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats. in Indian Journal of Experimental Biology. 2012;50(11):771-775. https://hdl.handle.net/21.15107/rcub_vinar_5153 .
Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats" in Indian Journal of Experimental Biology, 50, no. 11 (2012):771-775, https://hdl.handle.net/21.15107/rcub_vinar_5153 .