Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats
Authorized Users Only
2012
Authors
Đorđević, Ana D.Đorđević, Jelena D.
Elaković, Ivana
Adžić, Miroslav
Matić, Gordana
Radoičić, Marija B.
Review (Published version)
Metadata
Show full item recordAbstract
Plastic response and successful adaptation to stress are of particular importance in the hippocampus, where chronic stress may cause cell death instead of neural remodeling. Structural modifications that occur both in the brain of depressed patients and animal stress models may be reversed by antidepressants. Since morphological changes induced by stress and/or antidepressants could be mediated by presynaptically located proteins, determining the levels of these proteins may be a useful way to identify molecular changes associated with synaptic plasticity. In this study we analyzed the effects of chronic (six-week) social isolation and long-term (three-week) fluoxetine treatment on molecular markers of plasticity and apoptosis in the hippocampus of Wistar rats. Compartmental redistribution of NF kappa B transcription factor involved in the regulation of plasticity and apoptosis was also examined. To establish whether social isolation is able to evoke behavioral-like effects, which migh...t be related to the observed molecular changes, we performed the forced swimming test. The results show that synaptosomal polysialic neural cell adhesion molecule (PSA-NCAM), a molecular plasticity marker, was increased in the hippocampus of chronically isolated rats, while subsequent treatment with fluoxetine set it at the control level. In addition, analysis of cytoplasm/mitochondria redistribution of apoptotic proteins Bax and Bcl-2 after exposure to chronic isolation stress, revealed an increase in Bcl-2 protein expression in both compartments, while fluoxetine enhanced the effect of stress only in the mitochondria. The observed alterations at the molecular level were accompanied by normalization of stress-induced behavioral changes by fluoxetine. (C) 2011 Elsevier Inc. All rights reserved.
Keywords:
Antidepressant fluoxetine / Apoptosis / Forced swimming test / Hippocampus / Plasticity / StressSource:
Progress in Neuro-psychopharmacology and Biological Psychiatry, 2012, 36, 1, 92-100Funding / projects:
- Defining a cluster of molecular biomarkers for improved diagnostics and therapy of mood disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1016/j.pnpbp.2011.10.006
ISSN: 0278-5846; 1878-4216
PubMed: 22019604
WoS: 000300073000015
Scopus: 2-s2.0-82555170339
Collections
Institution/Community
VinčaTY - JOUR AU - Đorđević, Ana D. AU - Đorđević, Jelena D. AU - Elaković, Ivana AU - Adžić, Miroslav AU - Matić, Gordana AU - Radoičić, Marija B. PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4678 AB - Plastic response and successful adaptation to stress are of particular importance in the hippocampus, where chronic stress may cause cell death instead of neural remodeling. Structural modifications that occur both in the brain of depressed patients and animal stress models may be reversed by antidepressants. Since morphological changes induced by stress and/or antidepressants could be mediated by presynaptically located proteins, determining the levels of these proteins may be a useful way to identify molecular changes associated with synaptic plasticity. In this study we analyzed the effects of chronic (six-week) social isolation and long-term (three-week) fluoxetine treatment on molecular markers of plasticity and apoptosis in the hippocampus of Wistar rats. Compartmental redistribution of NF kappa B transcription factor involved in the regulation of plasticity and apoptosis was also examined. To establish whether social isolation is able to evoke behavioral-like effects, which might be related to the observed molecular changes, we performed the forced swimming test. The results show that synaptosomal polysialic neural cell adhesion molecule (PSA-NCAM), a molecular plasticity marker, was increased in the hippocampus of chronically isolated rats, while subsequent treatment with fluoxetine set it at the control level. In addition, analysis of cytoplasm/mitochondria redistribution of apoptotic proteins Bax and Bcl-2 after exposure to chronic isolation stress, revealed an increase in Bcl-2 protein expression in both compartments, while fluoxetine enhanced the effect of stress only in the mitochondria. The observed alterations at the molecular level were accompanied by normalization of stress-induced behavioral changes by fluoxetine. (C) 2011 Elsevier Inc. All rights reserved. T2 - Progress in Neuro-psychopharmacology and Biological Psychiatry T1 - Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats VL - 36 IS - 1 SP - 92 EP - 100 DO - 10.1016/j.pnpbp.2011.10.006 ER -
@article{ author = "Đorđević, Ana D. and Đorđević, Jelena D. and Elaković, Ivana and Adžić, Miroslav and Matić, Gordana and Radoičić, Marija B.", year = "2012", abstract = "Plastic response and successful adaptation to stress are of particular importance in the hippocampus, where chronic stress may cause cell death instead of neural remodeling. Structural modifications that occur both in the brain of depressed patients and animal stress models may be reversed by antidepressants. Since morphological changes induced by stress and/or antidepressants could be mediated by presynaptically located proteins, determining the levels of these proteins may be a useful way to identify molecular changes associated with synaptic plasticity. In this study we analyzed the effects of chronic (six-week) social isolation and long-term (three-week) fluoxetine treatment on molecular markers of plasticity and apoptosis in the hippocampus of Wistar rats. Compartmental redistribution of NF kappa B transcription factor involved in the regulation of plasticity and apoptosis was also examined. To establish whether social isolation is able to evoke behavioral-like effects, which might be related to the observed molecular changes, we performed the forced swimming test. The results show that synaptosomal polysialic neural cell adhesion molecule (PSA-NCAM), a molecular plasticity marker, was increased in the hippocampus of chronically isolated rats, while subsequent treatment with fluoxetine set it at the control level. In addition, analysis of cytoplasm/mitochondria redistribution of apoptotic proteins Bax and Bcl-2 after exposure to chronic isolation stress, revealed an increase in Bcl-2 protein expression in both compartments, while fluoxetine enhanced the effect of stress only in the mitochondria. The observed alterations at the molecular level were accompanied by normalization of stress-induced behavioral changes by fluoxetine. (C) 2011 Elsevier Inc. All rights reserved.", journal = "Progress in Neuro-psychopharmacology and Biological Psychiatry", title = "Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats", volume = "36", number = "1", pages = "92-100", doi = "10.1016/j.pnpbp.2011.10.006" }
Đorđević, A. D., Đorđević, J. D., Elaković, I., Adžić, M., Matić, G.,& Radoičić, M. B.. (2012). Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats. in Progress in Neuro-psychopharmacology and Biological Psychiatry, 36(1), 92-100. https://doi.org/10.1016/j.pnpbp.2011.10.006
Đorđević AD, Đorđević JD, Elaković I, Adžić M, Matić G, Radoičić MB. Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats. in Progress in Neuro-psychopharmacology and Biological Psychiatry. 2012;36(1):92-100. doi:10.1016/j.pnpbp.2011.10.006 .
Đorđević, Ana D., Đorđević, Jelena D., Elaković, Ivana, Adžić, Miroslav, Matić, Gordana, Radoičić, Marija B., "Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats" in Progress in Neuro-psychopharmacology and Biological Psychiatry, 36, no. 1 (2012):92-100, https://doi.org/10.1016/j.pnpbp.2011.10.006 . .