Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles
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Autori
Vujačić, Ana V.Vodnik, Vesna
Joksić, Gordana
Petrović, Sandra
Leskovac, Andreja
Nastasijević, Branislav J.
Vasić, Vesna M.
Članak u časopisu
Metapodaci
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The spherical gold nanoparticles (NPs) of different average size (15 nm and 47 nm) prepared by citrate reduction were used to examine their cytotoxic effects. UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements were used to characterize the gold NPs. We have investigated the concentration and size dependent cytotoxic effects of gold NPs, using two in vitro human cells model systems: proliferating lymphocytes and connective tissue fibroblasts. Treatment of lymphocyte cultures with Au NPs caused cytotoxic effects as revealed by significant enhancement of incidence of micronuclei but non significant increase of cell proliferation potential when compared to the control. In a fibroblast cell line, the same doses of gold NPs induced the slightly higher level of gamma-H2AX foci than in a control. The presented results clearly indicate that the enhancement of micronuclei incidence and proliferation index depend not on...ly on concentration but also the size of the nanoparticles.
Ključne reči:
gold / human cells / nanoparticles / cytotoxicityIzvor:
Digest Journal of Nanomaterials and Biostructures, 2011, 6, 3, 1367-1376Finansiranje / projekti:
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-MESTD-Basic Research (BR or ON)-172023)
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Institucija/grupa
VinčaTY - JOUR AU - Vujačić, Ana V. AU - Vodnik, Vesna AU - Joksić, Gordana AU - Petrović, Sandra AU - Leskovac, Andreja AU - Nastasijević, Branislav J. AU - Vasić, Vesna M. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4606 AB - The spherical gold nanoparticles (NPs) of different average size (15 nm and 47 nm) prepared by citrate reduction were used to examine their cytotoxic effects. UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements were used to characterize the gold NPs. We have investigated the concentration and size dependent cytotoxic effects of gold NPs, using two in vitro human cells model systems: proliferating lymphocytes and connective tissue fibroblasts. Treatment of lymphocyte cultures with Au NPs caused cytotoxic effects as revealed by significant enhancement of incidence of micronuclei but non significant increase of cell proliferation potential when compared to the control. In a fibroblast cell line, the same doses of gold NPs induced the slightly higher level of gamma-H2AX foci than in a control. The presented results clearly indicate that the enhancement of micronuclei incidence and proliferation index depend not only on concentration but also the size of the nanoparticles. T2 - Digest Journal of Nanomaterials and Biostructures T1 - Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles VL - 6 IS - 3 SP - 1367 EP - 1376 UR - https://hdl.handle.net/21.15107/rcub_vinar_4606 ER -
@article{ author = "Vujačić, Ana V. and Vodnik, Vesna and Joksić, Gordana and Petrović, Sandra and Leskovac, Andreja and Nastasijević, Branislav J. and Vasić, Vesna M.", year = "2011", abstract = "The spherical gold nanoparticles (NPs) of different average size (15 nm and 47 nm) prepared by citrate reduction were used to examine their cytotoxic effects. UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements were used to characterize the gold NPs. We have investigated the concentration and size dependent cytotoxic effects of gold NPs, using two in vitro human cells model systems: proliferating lymphocytes and connective tissue fibroblasts. Treatment of lymphocyte cultures with Au NPs caused cytotoxic effects as revealed by significant enhancement of incidence of micronuclei but non significant increase of cell proliferation potential when compared to the control. In a fibroblast cell line, the same doses of gold NPs induced the slightly higher level of gamma-H2AX foci than in a control. The presented results clearly indicate that the enhancement of micronuclei incidence and proliferation index depend not only on concentration but also the size of the nanoparticles.", journal = "Digest Journal of Nanomaterials and Biostructures", title = "Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles", volume = "6", number = "3", pages = "1367-1376", url = "https://hdl.handle.net/21.15107/rcub_vinar_4606" }
Vujačić, A. V., Vodnik, V., Joksić, G., Petrović, S., Leskovac, A., Nastasijević, B. J.,& Vasić, V. M.. (2011). Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles. in Digest Journal of Nanomaterials and Biostructures, 6(3), 1367-1376. https://hdl.handle.net/21.15107/rcub_vinar_4606
Vujačić AV, Vodnik V, Joksić G, Petrović S, Leskovac A, Nastasijević BJ, Vasić VM. Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles. in Digest Journal of Nanomaterials and Biostructures. 2011;6(3):1367-1376. https://hdl.handle.net/21.15107/rcub_vinar_4606 .
Vujačić, Ana V., Vodnik, Vesna, Joksić, Gordana, Petrović, Sandra, Leskovac, Andreja, Nastasijević, Branislav J., Vasić, Vesna M., "Particle Size and Concentration Dependent Cytotoxicity of Citrate Capped Gold Nanoparticles" in Digest Journal of Nanomaterials and Biostructures, 6, no. 3 (2011):1367-1376, https://hdl.handle.net/21.15107/rcub_vinar_4606 .