Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid
Нема приказа
Аутори
Čolović, Mirjana B.Bajuk-Bogdanović, Danica V.
Avramović, Nataša
Holclajtner-Antunović, Ivanka D.
Bošnjaković-Pavlović, Nada
Vasić, Vesna M.
Krstić, Danijela Z.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
C. The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 x 2H(2)O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 x 10(-6)/4.3 x 10(-6), 2.9 x 10(-6)/3.1 x 10(-6) and 1.3 x 10(-3)/1.5 x 10(-3) for WSiA, WPA and Na2WO4 x 2H(2)O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 x 2H(2)O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 x 10(-3) mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 x 10(-6) for WSiA and 1.6 x 10(-6) f...or WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 x 2H(2)O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 x 2H(2)O/WSiA ( LT = 1 x 10(-3)/ 4 x 10(-6) mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors. (C) 2011 Elsevier Ltd. All rights reserved.
Кључне речи:
12-Tungstosilicic acid / 12-tungstophosphoric acid / Monolacunary Keggin anion / Na2WO4 x 2H(2)O / Na+/K+-ATPase / Ecto-nucleoside triphosphate diphosphohydrolases / Simultaneous exposureИзвор:
Bioorganic and Medicinal Chemistry, 2011, 19, 23, 7063-7069Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-172023)
- Електропроводни и редокс-активни полимери и олигомери: синтеза, структура, својства и примена (RS-172043)
DOI: 10.1016/j.bmc.2011.10.008
ISSN: 0968-0896
PubMed: 22047804
WoS: 000296752300012
Scopus: 2-s2.0-80655123135
Колекције
Институција/група
VinčaTY - JOUR AU - Čolović, Mirjana B. AU - Bajuk-Bogdanović, Danica V. AU - Avramović, Nataša AU - Holclajtner-Antunović, Ivanka D. AU - Bošnjaković-Pavlović, Nada AU - Vasić, Vesna M. AU - Krstić, Danijela Z. PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4558 AB - C. The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 x 2H(2)O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 x 10(-6)/4.3 x 10(-6), 2.9 x 10(-6)/3.1 x 10(-6) and 1.3 x 10(-3)/1.5 x 10(-3) for WSiA, WPA and Na2WO4 x 2H(2)O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 x 2H(2)O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 x 10(-3) mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 x 10(-6) for WSiA and 1.6 x 10(-6) for WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 x 2H(2)O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 x 2H(2)O/WSiA ( LT = 1 x 10(-3)/ 4 x 10(-6) mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors. (C) 2011 Elsevier Ltd. All rights reserved. T2 - Bioorganic and Medicinal Chemistry T1 - Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid VL - 19 IS - 23 SP - 7063 EP - 7069 DO - 10.1016/j.bmc.2011.10.008 ER -
@article{ author = "Čolović, Mirjana B. and Bajuk-Bogdanović, Danica V. and Avramović, Nataša and Holclajtner-Antunović, Ivanka D. and Bošnjaković-Pavlović, Nada and Vasić, Vesna M. and Krstić, Danijela Z.", year = "2011", abstract = "C. The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 x 2H(2)O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 x 10(-6)/4.3 x 10(-6), 2.9 x 10(-6)/3.1 x 10(-6) and 1.3 x 10(-3)/1.5 x 10(-3) for WSiA, WPA and Na2WO4 x 2H(2)O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 x 2H(2)O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 x 10(-3) mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 x 10(-6) for WSiA and 1.6 x 10(-6) for WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 x 2H(2)O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 x 2H(2)O/WSiA ( LT = 1 x 10(-3)/ 4 x 10(-6) mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors. (C) 2011 Elsevier Ltd. All rights reserved.", journal = "Bioorganic and Medicinal Chemistry", title = "Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid", volume = "19", number = "23", pages = "7063-7069", doi = "10.1016/j.bmc.2011.10.008" }
Čolović, M. B., Bajuk-Bogdanović, D. V., Avramović, N., Holclajtner-Antunović, I. D., Bošnjaković-Pavlović, N., Vasić, V. M.,& Krstić, D. Z.. (2011). Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid. in Bioorganic and Medicinal Chemistry, 19(23), 7063-7069. https://doi.org/10.1016/j.bmc.2011.10.008
Čolović MB, Bajuk-Bogdanović DV, Avramović N, Holclajtner-Antunović ID, Bošnjaković-Pavlović N, Vasić VM, Krstić DZ. Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid. in Bioorganic and Medicinal Chemistry. 2011;19(23):7063-7069. doi:10.1016/j.bmc.2011.10.008 .
Čolović, Mirjana B., Bajuk-Bogdanović, Danica V., Avramović, Nataša, Holclajtner-Antunović, Ivanka D., Bošnjaković-Pavlović, Nada, Vasić, Vesna M., Krstić, Danijela Z., "Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid" in Bioorganic and Medicinal Chemistry, 19, no. 23 (2011):7063-7069, https://doi.org/10.1016/j.bmc.2011.10.008 . .