Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)
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Аутори
Kamceva, TinaFlemmig, Joerg
Damnjanović, Bojana
Arnhold, Juergen
Mijatovic, Aleksandar
Petković, Marijana
Чланак у часопису
Метаподаци
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Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.
Извор:
Metallomics, 2011, 3, 10, 1056-1063Финансирање / пројекти:
- Ministry of Sciences and Technological Development of the Republic of Serbia [127011], German Academic Exchange Service [50427034]
DOI: 10.1039/c1mt00088h
ISSN: 1756-5901
PubMed: 21909579
WoS: 000295581700013
Scopus: 2-s2.0-80053607258
Колекције
Институција/група
VinčaTY - JOUR AU - Kamceva, Tina AU - Flemmig, Joerg AU - Damnjanović, Bojana AU - Arnhold, Juergen AU - Mijatovic, Aleksandar AU - Petković, Marijana PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4514 AB - Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed. T2 - Metallomics T1 - Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2) VL - 3 IS - 10 SP - 1056 EP - 1063 DO - 10.1039/c1mt00088h ER -
@article{ author = "Kamceva, Tina and Flemmig, Joerg and Damnjanović, Bojana and Arnhold, Juergen and Mijatovic, Aleksandar and Petković, Marijana", year = "2011", abstract = "Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.", journal = "Metallomics", title = "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)", volume = "3", number = "10", pages = "1056-1063", doi = "10.1039/c1mt00088h" }
Kamceva, T., Flemmig, J., Damnjanović, B., Arnhold, J., Mijatovic, A.,& Petković, M.. (2011). Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics, 3(10), 1056-1063. https://doi.org/10.1039/c1mt00088h
Kamceva T, Flemmig J, Damnjanović B, Arnhold J, Mijatovic A, Petković M. Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics. 2011;3(10):1056-1063. doi:10.1039/c1mt00088h .
Kamceva, Tina, Flemmig, Joerg, Damnjanović, Bojana, Arnhold, Juergen, Mijatovic, Aleksandar, Petković, Marijana, "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)" in Metallomics, 3, no. 10 (2011):1056-1063, https://doi.org/10.1039/c1mt00088h . .