The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide
2011
Аутори
Dikic, DragoslavaBudec, Mirela
Vranješ-Đurić, Sanja
Koko, Vesna
Vignjevic, Sanja
Mitrovic, Olivera
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in ab...solute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.
Извор:
Alcohol and Alcoholism, 2011, 46, 5, 523-528Финансирање / пројекти:
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
DOI: 10.1093/alcalc/agr054
ISSN: 0735-0414
PubMed: 21610084
WoS: 000294066300003
Scopus: 2-s2.0-80051978015
Колекције
Институција/група
VinčaTY - JOUR AU - Dikic, Dragoslava AU - Budec, Mirela AU - Vranješ-Đurić, Sanja AU - Koko, Vesna AU - Vignjevic, Sanja AU - Mitrovic, Olivera PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4457 AB - Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions. T2 - Alcohol and Alcoholism T1 - The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide VL - 46 IS - 5 SP - 523 EP - 528 DO - 10.1093/alcalc/agr054 ER -
@article{ author = "Dikic, Dragoslava and Budec, Mirela and Vranješ-Đurić, Sanja and Koko, Vesna and Vignjevic, Sanja and Mitrovic, Olivera", year = "2011", abstract = "Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.", journal = "Alcohol and Alcoholism", title = "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide", volume = "46", number = "5", pages = "523-528", doi = "10.1093/alcalc/agr054" }
Dikic, D., Budec, M., Vranješ-Đurić, S., Koko, V., Vignjevic, S.,& Mitrovic, O.. (2011). The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol and Alcoholism, 46(5), 523-528. https://doi.org/10.1093/alcalc/agr054
Dikic D, Budec M, Vranješ-Đurić S, Koko V, Vignjevic S, Mitrovic O. The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol and Alcoholism. 2011;46(5):523-528. doi:10.1093/alcalc/agr054 .
Dikic, Dragoslava, Budec, Mirela, Vranješ-Đurić, Sanja, Koko, Vesna, Vignjevic, Sanja, Mitrovic, Olivera, "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide" in Alcohol and Alcoholism, 46, no. 5 (2011):523-528, https://doi.org/10.1093/alcalc/agr054 . .