Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes
Nema prikaza
Autori
Stanojević, IvanaBjelobaba, Ivana
Nedeljković, Nadežda
Drakulić, Dunja R.
Petrović, Snježana
Stojiljković, Mirjana
Horvat, Anica
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during po...stnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.
Ključne reči:
Ecto-5 -nucleotidase / AMP hydrolysis / Synaptosomes / Synaptic plasma membranes / Ontogeny profile / Rat brainIzvor:
International Journal of Developmental Neuroscience, 2011, 29, 4, 397-403Finansiranje / projekti:
- Molekularni mehanizmi patofizioloških promena u ćelijama centralnog nervnog sistema i perifernog tkiva kod sisara (RS-MESTD-Basic Research (BR or ON)-173044)
- Ćelijska i molekulska osnova neuroinflamacije: potencijala ciljna mesta za translacionu medicinu i terapiju (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.1016/j.ijdevneu.2011.03.003
ISSN: 0736-5748
PubMed: 21414400
WoS: 000291506000006
Scopus: 2-s2.0-79955784396
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Stanojević, Ivana AU - Bjelobaba, Ivana AU - Nedeljković, Nadežda AU - Drakulić, Dunja R. AU - Petrović, Snježana AU - Stojiljković, Mirjana AU - Horvat, Anica PY - 2011 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4376 AB - Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved. T2 - International Journal of Developmental Neuroscience T1 - Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes VL - 29 IS - 4 SP - 397 EP - 403 DO - 10.1016/j.ijdevneu.2011.03.003 ER -
@article{ author = "Stanojević, Ivana and Bjelobaba, Ivana and Nedeljković, Nadežda and Drakulić, Dunja R. and Petrović, Snježana and Stojiljković, Mirjana and Horvat, Anica", year = "2011", abstract = "Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.", journal = "International Journal of Developmental Neuroscience", title = "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes", volume = "29", number = "4", pages = "397-403", doi = "10.1016/j.ijdevneu.2011.03.003" }
Stanojević, I., Bjelobaba, I., Nedeljković, N., Drakulić, D. R., Petrović, S., Stojiljković, M.,& Horvat, A.. (2011). Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience, 29(4), 397-403. https://doi.org/10.1016/j.ijdevneu.2011.03.003
Stanojević I, Bjelobaba I, Nedeljković N, Drakulić DR, Petrović S, Stojiljković M, Horvat A. Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience. 2011;29(4):397-403. doi:10.1016/j.ijdevneu.2011.03.003 .
Stanojević, Ivana, Bjelobaba, Ivana, Nedeljković, Nadežda, Drakulić, Dunja R., Petrović, Snježana, Stojiljković, Mirjana, Horvat, Anica, "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes" in International Journal of Developmental Neuroscience, 29, no. 4 (2011):397-403, https://doi.org/10.1016/j.ijdevneu.2011.03.003 . .