Chronic isolation stress compromises JNK/c-Jun signaling in rat brain
Apstrakt
The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the h...ippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.
Ključne reči:
JNKs / c-Jun / Chronic stress / Rat brainIzvor:
Journal of Neural Transmission, 2012, 119, 11, 1275-1284Finansiranje / projekti:
DOI: 10.1007/s00702-012-0776-0
ISSN: 0300-9564
PubMed: 22358066
WoS: 000310086500004
Scopus: 2-s2.0-84869508743
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Filipović, Dragana AU - Zlatković, Jelena AU - Pavicevic, Ivan AU - Mandic, Ljuba AU - Demajo, Miroslav PY - 2012 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4360 AB - The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling. T2 - Journal of Neural Transmission T1 - Chronic isolation stress compromises JNK/c-Jun signaling in rat brain VL - 119 IS - 11 SP - 1275 EP - 1284 DO - 10.1007/s00702-012-0776-0 ER -
@article{ author = "Filipović, Dragana and Zlatković, Jelena and Pavicevic, Ivan and Mandic, Ljuba and Demajo, Miroslav", year = "2012", abstract = "The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.", journal = "Journal of Neural Transmission", title = "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain", volume = "119", number = "11", pages = "1275-1284", doi = "10.1007/s00702-012-0776-0" }
Filipović, D., Zlatković, J., Pavicevic, I., Mandic, L.,& Demajo, M.. (2012). Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission, 119(11), 1275-1284. https://doi.org/10.1007/s00702-012-0776-0
Filipović D, Zlatković J, Pavicevic I, Mandic L, Demajo M. Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission. 2012;119(11):1275-1284. doi:10.1007/s00702-012-0776-0 .
Filipović, Dragana, Zlatković, Jelena, Pavicevic, Ivan, Mandic, Ljuba, Demajo, Miroslav, "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain" in Journal of Neural Transmission, 119, no. 11 (2012):1275-1284, https://doi.org/10.1007/s00702-012-0776-0 . .