Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors
Апстракт
Human cytomegalovirus (HCMV) establishes a life-long persistent infection. HCMV infection could be associated with chronic inflammatory diseases, such as cardiovascular disease and atherosclerosis. Here we observed that in HCMV (AD-169) pre-exposed human umbilical vein endothelial cells (HUVEC), thrombin-induced expression of IL-1 alpha and M-CSF is markedly enhanced compared to the un-exposed cells. Study of the expression of thrombin receptor genes in HUVEC showed that HCMV triggered a time- and concentration-dependent expression of the thrombin receptors PAR1, PAR3 and PAR4 at the mRNA level. Induction of PAR1 and PAR3 mRNA expression is due to transcriptional activation of their promoters as shown by gene reporter assay. Furthermore, the virus induced expression of PAR1 and PAR3 but not PAR4 proteins, as analyzed by Western immunoblotting. However, flow cytometric analysis revealed that only PAR3, expressed at very low level in control HUVEC, is induced at the surface during the ex...posure to the virus. Our data suggest that although exposure to HCMV induces a minor increase of cell-surface receptors expression, it does make endothelial cells more responsive to additional thrombin stimulation.
Кључне речи:
HCMV / Thrombin / HUVEC / PAR1 / PAR3 / IL-1 alpha / M-CSFИзвор:
Journal of Thrombosis and Thrombolysis, 2010, 30, 2, 164-171Финансирање / пројекти:
- Deutsche Forschungsgemeinschaft, Serbian Government [M145023]
DOI: 10.1007/s11239-010-0447-7
ISSN: 0929-5305
PubMed: 20155436
WoS: 000280262500007
Scopus: 2-s2.0-77957958031
Колекције
Институција/група
VinčaTY - JOUR AU - Popović, Milan AU - Paskas, Svetlana AU - Živković, Maja AU - Burysek, Ladislav AU - Laumonnier, Yves PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4059 AB - Human cytomegalovirus (HCMV) establishes a life-long persistent infection. HCMV infection could be associated with chronic inflammatory diseases, such as cardiovascular disease and atherosclerosis. Here we observed that in HCMV (AD-169) pre-exposed human umbilical vein endothelial cells (HUVEC), thrombin-induced expression of IL-1 alpha and M-CSF is markedly enhanced compared to the un-exposed cells. Study of the expression of thrombin receptor genes in HUVEC showed that HCMV triggered a time- and concentration-dependent expression of the thrombin receptors PAR1, PAR3 and PAR4 at the mRNA level. Induction of PAR1 and PAR3 mRNA expression is due to transcriptional activation of their promoters as shown by gene reporter assay. Furthermore, the virus induced expression of PAR1 and PAR3 but not PAR4 proteins, as analyzed by Western immunoblotting. However, flow cytometric analysis revealed that only PAR3, expressed at very low level in control HUVEC, is induced at the surface during the exposure to the virus. Our data suggest that although exposure to HCMV induces a minor increase of cell-surface receptors expression, it does make endothelial cells more responsive to additional thrombin stimulation. T2 - Journal of Thrombosis and Thrombolysis T1 - Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors VL - 30 IS - 2 SP - 164 EP - 171 DO - 10.1007/s11239-010-0447-7 ER -
@article{ author = "Popović, Milan and Paskas, Svetlana and Živković, Maja and Burysek, Ladislav and Laumonnier, Yves", year = "2010", abstract = "Human cytomegalovirus (HCMV) establishes a life-long persistent infection. HCMV infection could be associated with chronic inflammatory diseases, such as cardiovascular disease and atherosclerosis. Here we observed that in HCMV (AD-169) pre-exposed human umbilical vein endothelial cells (HUVEC), thrombin-induced expression of IL-1 alpha and M-CSF is markedly enhanced compared to the un-exposed cells. Study of the expression of thrombin receptor genes in HUVEC showed that HCMV triggered a time- and concentration-dependent expression of the thrombin receptors PAR1, PAR3 and PAR4 at the mRNA level. Induction of PAR1 and PAR3 mRNA expression is due to transcriptional activation of their promoters as shown by gene reporter assay. Furthermore, the virus induced expression of PAR1 and PAR3 but not PAR4 proteins, as analyzed by Western immunoblotting. However, flow cytometric analysis revealed that only PAR3, expressed at very low level in control HUVEC, is induced at the surface during the exposure to the virus. Our data suggest that although exposure to HCMV induces a minor increase of cell-surface receptors expression, it does make endothelial cells more responsive to additional thrombin stimulation.", journal = "Journal of Thrombosis and Thrombolysis", title = "Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors", volume = "30", number = "2", pages = "164-171", doi = "10.1007/s11239-010-0447-7" }
Popović, M., Paskas, S., Živković, M., Burysek, L.,& Laumonnier, Y.. (2010). Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors. in Journal of Thrombosis and Thrombolysis, 30(2), 164-171. https://doi.org/10.1007/s11239-010-0447-7
Popović M, Paskas S, Živković M, Burysek L, Laumonnier Y. Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors. in Journal of Thrombosis and Thrombolysis. 2010;30(2):164-171. doi:10.1007/s11239-010-0447-7 .
Popović, Milan, Paskas, Svetlana, Živković, Maja, Burysek, Ladislav, Laumonnier, Yves, "Human cytomegalovirus increases HUVEC sensitivity to thrombin and modulates expression of thrombin receptors" in Journal of Thrombosis and Thrombolysis, 30, no. 2 (2010):164-171, https://doi.org/10.1007/s11239-010-0447-7 . .