Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping
Abstract
Background:Numerous studies have reported a relationship betweenhepatitis C virus (HCV) genotype and the response to interferon therapy.Despite high sensitivity and specificity, genotyping methods can be per-formed only on HCV RNA positive samples. Serotyping might be a rapidand cost effective method for determining HCV genotypes, especially inpatients with previously undetectable HCV RNA.Aim:The aim of this study was to select NS5A-derived peptides whichmay represent candidate specific markers for determination of HCVgenotypes by serotyping.Methods:The conserved structural and informational properties of HCVNS5A protein have been determined by the informational spectrum method(ISM), representing virtual spectroscopy method for analysis of relationshipbetween structure and function of proteins.Results:The ISM analysis revealed that NS5A proteins from HCV geno-types 1,2,3,4 and 6 encode the conserved information which is specific foreasch particular genotype, and which is represented by... IS frequenciesF1(0.258), F2(0.271), F3(0.053), F4(0.205) and F6(0.146), respectively. TheNS5-derived peptides P1(120-152), P2(383-417), P3(405-439), P4(171-205), P6(374-408) are identified as domains that are essential for conservedinformation characterizing HCV genotypes 1, 2, 3, 4 and 6.Conclusions:Peptides P1, P2, P3, P4 and P6 derived from NS5A proteinrepresent candidate markers for simple and fast identification of HCVgenotypes by serotyping
Source:
Vox Sanguinis, 2010, 99, Supp: 1, 299-Collections
Institution/Community
VinčaTY - CONF AU - Vasiljević, N. AU - Veljković, Nevena V. AU - Jovanović-Ćupić, Snežana P. AU - Glišić, Sanja PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/4043 AB - Background:Numerous studies have reported a relationship betweenhepatitis C virus (HCV) genotype and the response to interferon therapy.Despite high sensitivity and specificity, genotyping methods can be per-formed only on HCV RNA positive samples. Serotyping might be a rapidand cost effective method for determining HCV genotypes, especially inpatients with previously undetectable HCV RNA.Aim:The aim of this study was to select NS5A-derived peptides whichmay represent candidate specific markers for determination of HCVgenotypes by serotyping.Methods:The conserved structural and informational properties of HCVNS5A protein have been determined by the informational spectrum method(ISM), representing virtual spectroscopy method for analysis of relationshipbetween structure and function of proteins.Results:The ISM analysis revealed that NS5A proteins from HCV geno-types 1,2,3,4 and 6 encode the conserved information which is specific foreasch particular genotype, and which is represented by IS frequenciesF1(0.258), F2(0.271), F3(0.053), F4(0.205) and F6(0.146), respectively. TheNS5-derived peptides P1(120-152), P2(383-417), P3(405-439), P4(171-205), P6(374-408) are identified as domains that are essential for conservedinformation characterizing HCV genotypes 1, 2, 3, 4 and 6.Conclusions:Peptides P1, P2, P3, P4 and P6 derived from NS5A proteinrepresent candidate markers for simple and fast identification of HCVgenotypes by serotyping C3 - Vox Sanguinis T1 - Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping VL - 99 IS - Supp: 1 SP - 299 DO - 10.1111/j.1423-0410.2010.01343_2.x UR - https://hdl.handle.net/21.15107/rcub_vinar_4043 ER -
@conference{ author = "Vasiljević, N. and Veljković, Nevena V. and Jovanović-Ćupić, Snežana P. and Glišić, Sanja", year = "2010", abstract = "Background:Numerous studies have reported a relationship betweenhepatitis C virus (HCV) genotype and the response to interferon therapy.Despite high sensitivity and specificity, genotyping methods can be per-formed only on HCV RNA positive samples. Serotyping might be a rapidand cost effective method for determining HCV genotypes, especially inpatients with previously undetectable HCV RNA.Aim:The aim of this study was to select NS5A-derived peptides whichmay represent candidate specific markers for determination of HCVgenotypes by serotyping.Methods:The conserved structural and informational properties of HCVNS5A protein have been determined by the informational spectrum method(ISM), representing virtual spectroscopy method for analysis of relationshipbetween structure and function of proteins.Results:The ISM analysis revealed that NS5A proteins from HCV geno-types 1,2,3,4 and 6 encode the conserved information which is specific foreasch particular genotype, and which is represented by IS frequenciesF1(0.258), F2(0.271), F3(0.053), F4(0.205) and F6(0.146), respectively. TheNS5-derived peptides P1(120-152), P2(383-417), P3(405-439), P4(171-205), P6(374-408) are identified as domains that are essential for conservedinformation characterizing HCV genotypes 1, 2, 3, 4 and 6.Conclusions:Peptides P1, P2, P3, P4 and P6 derived from NS5A proteinrepresent candidate markers for simple and fast identification of HCVgenotypes by serotyping", journal = "Vox Sanguinis", title = "Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping", volume = "99", number = "Supp: 1", pages = "299", doi = "10.1111/j.1423-0410.2010.01343_2.x", url = "https://hdl.handle.net/21.15107/rcub_vinar_4043" }
Vasiljević, N., Veljković, N. V., Jovanović-Ćupić, S. P.,& Glišić, S.. (2010). Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping. in Vox Sanguinis, 99(Supp: 1), 299. https://doi.org/10.1111/j.1423-0410.2010.01343_2.x https://hdl.handle.net/21.15107/rcub_vinar_4043
Vasiljević N, Veljković NV, Jovanović-Ćupić SP, Glišić S. Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping. in Vox Sanguinis. 2010;99(Supp: 1):299. doi:10.1111/j.1423-0410.2010.01343_2.x https://hdl.handle.net/21.15107/rcub_vinar_4043 .
Vasiljević, N., Veljković, Nevena V., Jovanović-Ćupić, Snežana P., Glišić, Sanja, "Selection of Ns5a-Derived Peptides for Determination of Hcv Genotypes By Serotyping" in Vox Sanguinis, 99, no. Supp: 1 (2010):299, https://doi.org/10.1111/j.1423-0410.2010.01343_2.x ., https://hdl.handle.net/21.15107/rcub_vinar_4043 .