The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro
2010
Authors
Bajić, Vladan P.Stanimirovic, Z.
Stevanović, Jevrosima
Milićević, Zorka T.
Živković, Lada
Spremo-Potparević, Biljana
Article
Metadata
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Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.
Keywords:
Paclitaxel (Ptx) / premature centromere division (PCD) / cycloheximide / cytokinesis block micronucleus assay (CBMN) / fluorescent in situ hybridization (FISH)Source:
Archives of Biological Sciences, 2010, 62, 1, 63-74Funding / projects:
- Serbian Ministry of Science and Technological Development [143022]
DOI: 10.2298/ABS1001063B
ISSN: 0354-4664
WoS: 000277610000009
Scopus: 2-s2.0-77955153267
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VinčaTY - JOUR AU - Bajić, Vladan P. AU - Stanimirovic, Z. AU - Stevanović, Jevrosima AU - Milićević, Zorka T. AU - Živković, Lada AU - Spremo-Potparević, Biljana PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3998 AB - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment. T2 - Archives of Biological Sciences T1 - The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro VL - 62 IS - 1 SP - 63 EP - 74 DO - 10.2298/ABS1001063B ER -
@article{ author = "Bajić, Vladan P. and Stanimirovic, Z. and Stevanović, Jevrosima and Milićević, Zorka T. and Živković, Lada and Spremo-Potparević, Biljana", year = "2010", abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.", journal = "Archives of Biological Sciences", title = "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro", volume = "62", number = "1", pages = "63-74", doi = "10.2298/ABS1001063B" }
Bajić, V. P., Stanimirovic, Z., Stevanović, J., Milićević, Z. T., Živković, L.,& Spremo-Potparević, B.. (2010). The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences, 62(1), 63-74. https://doi.org/10.2298/ABS1001063B
Bajić VP, Stanimirovic Z, Stevanović J, Milićević ZT, Živković L, Spremo-Potparević B. The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences. 2010;62(1):63-74. doi:10.2298/ABS1001063B .
Bajić, Vladan P., Stanimirovic, Z., Stevanović, Jevrosima, Milićević, Zorka T., Živković, Lada, Spremo-Potparević, Biljana, "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74, https://doi.org/10.2298/ABS1001063B . .