Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development
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Аутори
Horvat, AnicaStanojević, Ivana
Drakulić, Dunja R.
Velickovic, Natasa
Petrović, Snježana
Milošević, Maja
Чланак у часопису
Метаподаци
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The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased ...and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.
Кључне речи:
Acute restraint stress / NTPDase / Ecto-5 -nucleotidase / Postnatal development / Synaptosomal plasma membranes / Rat brainИзвор:
International Journal of Developmental Neuroscience, 2010, 28, 2, 175-182Финансирање / пројекти:
DOI: 10.1016/j.ijdevneu.2009.11.005
ISSN: 0736-5748
PubMed: 19932163
WoS: 000276381700009
Scopus: 2-s2.0-77649234625
Колекције
Институција/група
VinčaTY - JOUR AU - Horvat, Anica AU - Stanojević, Ivana AU - Drakulić, Dunja R. AU - Velickovic, Natasa AU - Petrović, Snježana AU - Milošević, Maja PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3941 AB - The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved. T2 - International Journal of Developmental Neuroscience T1 - Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development VL - 28 IS - 2 SP - 175 EP - 182 DO - 10.1016/j.ijdevneu.2009.11.005 ER -
@article{ author = "Horvat, Anica and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, Natasa and Petrović, Snježana and Milošević, Maja", year = "2010", abstract = "The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.", journal = "International Journal of Developmental Neuroscience", title = "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development", volume = "28", number = "2", pages = "175-182", doi = "10.1016/j.ijdevneu.2009.11.005" }
Horvat, A., Stanojević, I., Drakulić, D. R., Velickovic, N., Petrović, S.,& Milošević, M.. (2010). Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience, 28(2), 175-182. https://doi.org/10.1016/j.ijdevneu.2009.11.005
Horvat A, Stanojević I, Drakulić DR, Velickovic N, Petrović S, Milošević M. Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience. 2010;28(2):175-182. doi:10.1016/j.ijdevneu.2009.11.005 .
Horvat, Anica, Stanojević, Ivana, Drakulić, Dunja R., Velickovic, Natasa, Petrović, Snježana, Milošević, Maja, "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development" in International Journal of Developmental Neuroscience, 28, no. 2 (2010):175-182, https://doi.org/10.1016/j.ijdevneu.2009.11.005 . .