Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT
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Stanković, AleksandraŽivković, Maja
Kostić, Mirjana M.
Atanackovic, Jasmina
Krstić, Zoran
Alavantić, Dragan
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Objectives: Congenital anomalies of the kidney and urinary tract (CAKUT) are common causes of chronic renal failure in children. The angiotensin II receptor type 2 (AT2R) is one of proposed candidate genes for CAKUT, but the expression was never explored in humans. The aim was to establish the AM gene expression in human CAKUT concerning -1332A/G polymorphism, which might affect alternative splicing. Design and methods: Forty-eight patients with CAKUT constitute the basis of this study. Genotyping for -1332A/G, RT-PCR for AT2R gene expression and confirmation sequencing were performed. Results: The expression of Ex 1/2/3 and Ex 1/3 transcript splice variants of the AT2R mRNA were detected in human CAKUT tissue. The pattern was observed independently of A to G transition. Conclusions: The expression of AT2R mRNA in human CAKUT was established for the first time and was not affected by -1332A/G polymorphism in children with CAKUT. (c) 2009 The Canadian Society of Clinical Chemists. Publi...shed by Elsevier Inc. All rights reserved.
Keywords:
AT2R / Gene / mRNA / Expression / Polymorphism / CAKUTSource:
Clinical Biochemistry, 2010, 43, 1-2, 71-75Funding / projects:
- Serbian Government [M145023]
DOI: 10.1016/j.clinbiochem.2009.09.009
ISSN: 0009-9120; 1873-2933
PubMed: 19781541
WoS: 000273514400009
Scopus: 2-s2.0-72749113931
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VinčaTY - JOUR AU - Stanković, Aleksandra AU - Živković, Maja AU - Kostić, Mirjana M. AU - Atanackovic, Jasmina AU - Krstić, Zoran AU - Alavantić, Dragan PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3873 AB - Objectives: Congenital anomalies of the kidney and urinary tract (CAKUT) are common causes of chronic renal failure in children. The angiotensin II receptor type 2 (AT2R) is one of proposed candidate genes for CAKUT, but the expression was never explored in humans. The aim was to establish the AM gene expression in human CAKUT concerning -1332A/G polymorphism, which might affect alternative splicing. Design and methods: Forty-eight patients with CAKUT constitute the basis of this study. Genotyping for -1332A/G, RT-PCR for AT2R gene expression and confirmation sequencing were performed. Results: The expression of Ex 1/2/3 and Ex 1/3 transcript splice variants of the AT2R mRNA were detected in human CAKUT tissue. The pattern was observed independently of A to G transition. Conclusions: The expression of AT2R mRNA in human CAKUT was established for the first time and was not affected by -1332A/G polymorphism in children with CAKUT. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. T2 - Clinical Biochemistry T1 - Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT VL - 43 IS - 1-2 SP - 71 EP - 75 DO - 10.1016/j.clinbiochem.2009.09.009 ER -
@article{ author = "Stanković, Aleksandra and Živković, Maja and Kostić, Mirjana M. and Atanackovic, Jasmina and Krstić, Zoran and Alavantić, Dragan", year = "2010", abstract = "Objectives: Congenital anomalies of the kidney and urinary tract (CAKUT) are common causes of chronic renal failure in children. The angiotensin II receptor type 2 (AT2R) is one of proposed candidate genes for CAKUT, but the expression was never explored in humans. The aim was to establish the AM gene expression in human CAKUT concerning -1332A/G polymorphism, which might affect alternative splicing. Design and methods: Forty-eight patients with CAKUT constitute the basis of this study. Genotyping for -1332A/G, RT-PCR for AT2R gene expression and confirmation sequencing were performed. Results: The expression of Ex 1/2/3 and Ex 1/3 transcript splice variants of the AT2R mRNA were detected in human CAKUT tissue. The pattern was observed independently of A to G transition. Conclusions: The expression of AT2R mRNA in human CAKUT was established for the first time and was not affected by -1332A/G polymorphism in children with CAKUT. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.", journal = "Clinical Biochemistry", title = "Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT", volume = "43", number = "1-2", pages = "71-75", doi = "10.1016/j.clinbiochem.2009.09.009" }
Stanković, A., Živković, M., Kostić, M. M., Atanackovic, J., Krstić, Z.,& Alavantić, D.. (2010). Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT. in Clinical Biochemistry, 43(1-2), 71-75. https://doi.org/10.1016/j.clinbiochem.2009.09.009
Stanković A, Živković M, Kostić MM, Atanackovic J, Krstić Z, Alavantić D. Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT. in Clinical Biochemistry. 2010;43(1-2):71-75. doi:10.1016/j.clinbiochem.2009.09.009 .
Stanković, Aleksandra, Živković, Maja, Kostić, Mirjana M., Atanackovic, Jasmina, Krstić, Zoran, Alavantić, Dragan, "Expression profiling of the AT2R mRNA in affected tissue from children with CAKUT" in Clinical Biochemistry, 43, no. 1-2 (2010):71-75, https://doi.org/10.1016/j.clinbiochem.2009.09.009 . .