Concurrent quantitation of the A and D genotypes of hepatitis B virus
Нема приказа
Аутори
Tanić, NikolaStanojević, Boban
Tanić, Nikola
Schaefer, Stephan
Niesters, Hubert G. M.
Božić, Milena
Dimitrijević, Bogomir B.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of ...individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
Кључне речи:
Hepatitis B virus / Genotyping / Quantitation / Quantitative real time PCRИзвор:
Journal of Virological Methods, 2009, 161, 2, 265-270Финансирање / пројекти:
- Serbian Ministry of Science and Technology [143010]
DOI: 10.1016/j.jviromet.2009.06.022
ISSN: 0166-0934
PubMed: 19591875
WoS: 000269993000012
Scopus: 2-s2.0-68949215506
Колекције
Институција/група
VinčaTY - JOUR AU - Tanić, Nikola AU - Stanojević, Boban AU - Tanić, Nikola AU - Schaefer, Stephan AU - Niesters, Hubert G. M. AU - Božić, Milena AU - Dimitrijević, Bogomir B. PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3789 AB - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved. T2 - Journal of Virological Methods T1 - Concurrent quantitation of the A and D genotypes of hepatitis B virus VL - 161 IS - 2 SP - 265 EP - 270 DO - 10.1016/j.jviromet.2009.06.022 ER -
@article{ author = "Tanić, Nikola and Stanojević, Boban and Tanić, Nikola and Schaefer, Stephan and Niesters, Hubert G. M. and Božić, Milena and Dimitrijević, Bogomir B.", year = "2009", abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.", journal = "Journal of Virological Methods", title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus", volume = "161", number = "2", pages = "265-270", doi = "10.1016/j.jviromet.2009.06.022" }
Tanić, N., Stanojević, B., Tanić, N., Schaefer, S., Niesters, H. G. M., Božić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2), 265-270. https://doi.org/10.1016/j.jviromet.2009.06.022
Tanić N, Stanojević B, Tanić N, Schaefer S, Niesters HGM, Božić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):265-270. doi:10.1016/j.jviromet.2009.06.022 .
Tanić, Nikola, Stanojević, Boban, Tanić, Nikola, Schaefer, Stephan, Niesters, Hubert G. M., Božić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009):265-270, https://doi.org/10.1016/j.jviromet.2009.06.022 . .