Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids
Аутори
Petković, MarijanaSchiller, Jürgen
Mueller, Matthias
Suess, Rosmarie
Arnold, Klaus
Arnhold, Juergen
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.
Кључне речи:
Phospholipids / MALDI-TOF MS / Adducts / Matrix Clusters / Positive Ion Mode / Negative Ion ModeИзвор:
Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences, 2009, 64, 3, 331-334Финансирање / пројекти:
- German Research Council [Schi 476/5-1]
- Federal Ministry of Education and Research [0313836]
DOI: 10.1515/znb-2009-0314
ISSN: 0932-0776
WoS: 000268875000014
Scopus: 2-s2.0-62549090216
Колекције
Институција/група
VinčaTY - JOUR AU - Petković, Marijana AU - Schiller, Jürgen AU - Mueller, Matthias AU - Suess, Rosmarie AU - Arnold, Klaus AU - Arnhold, Juergen PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3770 AB - It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS. T2 - Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences T1 - Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids VL - 64 IS - 3 SP - 331 EP - 334 DO - 10.1515/znb-2009-0314 UR - https://hdl.handle.net/21.15107/rcub_vinar_3770 ER -
@article{ author = "Petković, Marijana and Schiller, Jürgen and Mueller, Matthias and Suess, Rosmarie and Arnold, Klaus and Arnhold, Juergen", year = "2009", abstract = "It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.", journal = "Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences", title = "Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids", volume = "64", number = "3", pages = "331-334", doi = "10.1515/znb-2009-0314", url = "https://hdl.handle.net/21.15107/rcub_vinar_3770" }
Petković, M., Schiller, J., Mueller, M., Suess, R., Arnold, K.,& Arnhold, J.. (2009). Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids. in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences, 64(3), 331-334. https://doi.org/10.1515/znb-2009-0314 https://hdl.handle.net/21.15107/rcub_vinar_3770
Petković M, Schiller J, Mueller M, Suess R, Arnold K, Arnhold J. Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids. in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences. 2009;64(3):331-334. doi:10.1515/znb-2009-0314 https://hdl.handle.net/21.15107/rcub_vinar_3770 .
Petković, Marijana, Schiller, Jürgen, Mueller, Matthias, Suess, Rosmarie, Arnold, Klaus, Arnhold, Juergen, "Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids" in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences, 64, no. 3 (2009):331-334, https://doi.org/10.1515/znb-2009-0314 ., https://hdl.handle.net/21.15107/rcub_vinar_3770 .