Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol
Аутори
Petrović, SnježanaMilošević, Maja
Stanojević, Ivana
Velickovic, Natasa
Drakulić, Dunja R.
Horvat, Anica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.
Кључне речи:
mitochondria / estradiol / Ca2+ influx / Ca2+ effluxИзвор:
Archives of Biological Sciences, 2009, 61, 2, 171-177Финансирање / пројекти:
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
Колекције
Институција/група
VinčaTY - JOUR AU - Petrović, Snježana AU - Milošević, Maja AU - Stanojević, Ivana AU - Velickovic, Natasa AU - Drakulić, Dunja R. AU - Horvat, Anica PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3749 AB - The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis. T2 - Archives of Biological Sciences T1 - Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol VL - 61 IS - 2 SP - 171 EP - 177 DO - 10.2298/ABS0902171P ER -
@article{ author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Velickovic, Natasa and Drakulić, Dunja R. and Horvat, Anica", year = "2009", abstract = "The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.", journal = "Archives of Biological Sciences", title = "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol", volume = "61", number = "2", pages = "171-177", doi = "10.2298/ABS0902171P" }
Petrović, S., Milošević, M., Stanojević, I., Velickovic, N., Drakulić, D. R.,& Horvat, A.. (2009). Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences, 61(2), 171-177. https://doi.org/10.2298/ABS0902171P
Petrović S, Milošević M, Stanojević I, Velickovic N, Drakulić DR, Horvat A. Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences. 2009;61(2):171-177. doi:10.2298/ABS0902171P .
Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Velickovic, Natasa, Drakulić, Dunja R., Horvat, Anica, "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol" in Archives of Biological Sciences, 61, no. 2 (2009):171-177, https://doi.org/10.2298/ABS0902171P . .