Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress
Abstract
Neuroendocrine stress (NES) causes increase of glucocorticoids and alters physiological levels of reactive oxygen species production in cells, which might involve modifications in the antioxidant defense system. We investigated the hypothesis that acute, chronic, or combined stress alters copper-zinc superoxide dismutase (CuZnSOD) expression pattern at both, mRNA and subcellular protein level in the cerebral cortex and hippocampus of rats and that there may be a relationship between stress-induced corticosterone and CuZnSOD expression. The most effective stress model which led to the most pronounced changes in CuZnSOD expression patterns was also investigated. Our results demonstrated that acute stress immobilization up-regulates mRNA expression of hippocampal CuZnSOD, while cytosolic protein expression of this enzyme was increased in both brain structures. Chronic stress isolation had no effect on either mRNA and protein expression level and caused a lack of significant up-regulation ...to a novel acute stressors. The presence of this protein in nuclear fractions of both brain structures was also confirmed. The elevated cytosolic CuZnSOD protein levels following acute immobilization might reflect on the defense system against oxidative stress. Chronic isolation compromises CuZnSOD protein expression, which may lead to the inefficient defense against reactive oxygen species (ROS). The stress-triggered CuZnSOD protein expression was not correlated by the corresponding mRNA. The results suggest that different stress models exert a different degree of influence on mRNA and protein level of CuZnSOD in both brain structures as well as serum corticosterone.
Keywords:
Cerebral cortex / Corticosterone / CuZn superoxide dismutase / Hippocampus / Neuroendocrine stressSource:
Cellular and Molecular Neurobiology, 2009, 29, 5, 673-681Funding / projects:
- Signalni putevi delovanja steroidnih hormona i uticaj endogenih i egzogenih faktora na modulaciju procesa u ćelijama sisara (RS-MESTD-MPN2006-2010-143044)
DOI: 10.1007/s10571-009-9375-5
ISSN: 0272-4340; 1573-6830
PubMed: 19283469
WoS: 000266983100007
Scopus: 2-s2.0-67650911578
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VinčaTY - JOUR AU - Filipović, Dragana AU - Pajović, Snežana B. PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3724 AB - Neuroendocrine stress (NES) causes increase of glucocorticoids and alters physiological levels of reactive oxygen species production in cells, which might involve modifications in the antioxidant defense system. We investigated the hypothesis that acute, chronic, or combined stress alters copper-zinc superoxide dismutase (CuZnSOD) expression pattern at both, mRNA and subcellular protein level in the cerebral cortex and hippocampus of rats and that there may be a relationship between stress-induced corticosterone and CuZnSOD expression. The most effective stress model which led to the most pronounced changes in CuZnSOD expression patterns was also investigated. Our results demonstrated that acute stress immobilization up-regulates mRNA expression of hippocampal CuZnSOD, while cytosolic protein expression of this enzyme was increased in both brain structures. Chronic stress isolation had no effect on either mRNA and protein expression level and caused a lack of significant up-regulation to a novel acute stressors. The presence of this protein in nuclear fractions of both brain structures was also confirmed. The elevated cytosolic CuZnSOD protein levels following acute immobilization might reflect on the defense system against oxidative stress. Chronic isolation compromises CuZnSOD protein expression, which may lead to the inefficient defense against reactive oxygen species (ROS). The stress-triggered CuZnSOD protein expression was not correlated by the corresponding mRNA. The results suggest that different stress models exert a different degree of influence on mRNA and protein level of CuZnSOD in both brain structures as well as serum corticosterone. T2 - Cellular and Molecular Neurobiology T1 - Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress VL - 29 IS - 5 SP - 673 EP - 681 DO - 10.1007/s10571-009-9375-5 ER -
@article{ author = "Filipović, Dragana and Pajović, Snežana B.", year = "2009", abstract = "Neuroendocrine stress (NES) causes increase of glucocorticoids and alters physiological levels of reactive oxygen species production in cells, which might involve modifications in the antioxidant defense system. We investigated the hypothesis that acute, chronic, or combined stress alters copper-zinc superoxide dismutase (CuZnSOD) expression pattern at both, mRNA and subcellular protein level in the cerebral cortex and hippocampus of rats and that there may be a relationship between stress-induced corticosterone and CuZnSOD expression. The most effective stress model which led to the most pronounced changes in CuZnSOD expression patterns was also investigated. Our results demonstrated that acute stress immobilization up-regulates mRNA expression of hippocampal CuZnSOD, while cytosolic protein expression of this enzyme was increased in both brain structures. Chronic stress isolation had no effect on either mRNA and protein expression level and caused a lack of significant up-regulation to a novel acute stressors. The presence of this protein in nuclear fractions of both brain structures was also confirmed. The elevated cytosolic CuZnSOD protein levels following acute immobilization might reflect on the defense system against oxidative stress. Chronic isolation compromises CuZnSOD protein expression, which may lead to the inefficient defense against reactive oxygen species (ROS). The stress-triggered CuZnSOD protein expression was not correlated by the corresponding mRNA. The results suggest that different stress models exert a different degree of influence on mRNA and protein level of CuZnSOD in both brain structures as well as serum corticosterone.", journal = "Cellular and Molecular Neurobiology", title = "Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress", volume = "29", number = "5", pages = "673-681", doi = "10.1007/s10571-009-9375-5" }
Filipović, D.,& Pajović, S. B.. (2009). Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress. in Cellular and Molecular Neurobiology, 29(5), 673-681. https://doi.org/10.1007/s10571-009-9375-5
Filipović D, Pajović SB. Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress. in Cellular and Molecular Neurobiology. 2009;29(5):673-681. doi:10.1007/s10571-009-9375-5 .
Filipović, Dragana, Pajović, Snežana B., "Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress" in Cellular and Molecular Neurobiology, 29, no. 5 (2009):673-681, https://doi.org/10.1007/s10571-009-9375-5 . .