The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles
Нема приказа
Аутори
Misirkić, Maja S.Todorović-Marković, Biljana
Vucicevic, Ljubica M.
Janjetović, Kristina D.
Jokanović, Vukoman R.
Dramićanin, Miroslav
Marković, Zoran M.
Trajković, Vladimir S.
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
The influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60... nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved.
Кључне речи:
Carbon / Nanoparticles / Nitric oxide / Cytotoxicity / ApoptosisИзвор:
Biomaterials, 2009, 30, 12, 2319-2328Финансирање / пројекти:
- Ministry of Science of the Republic of Serbia [145073]
DOI: 10.1016/j.biomaterials.2009.01.023
ISSN: 0142-9612
PubMed: 19195698
WoS: 000264512800017
Scopus: 2-s2.0-60649107790
Колекције
Институција/група
VinčaTY - JOUR AU - Misirkić, Maja S. AU - Todorović-Marković, Biljana AU - Vucicevic, Ljubica M. AU - Janjetović, Kristina D. AU - Jokanović, Vukoman R. AU - Dramićanin, Miroslav AU - Marković, Zoran M. AU - Trajković, Vladimir S. PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3659 AB - The influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60 nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved. T2 - Biomaterials T1 - The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles VL - 30 IS - 12 SP - 2319 EP - 2328 DO - 10.1016/j.biomaterials.2009.01.023 ER -
@article{ author = "Misirkić, Maja S. and Todorović-Marković, Biljana and Vucicevic, Ljubica M. and Janjetović, Kristina D. and Jokanović, Vukoman R. and Dramićanin, Miroslav and Marković, Zoran M. and Trajković, Vladimir S.", year = "2009", abstract = "The influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60 nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved.", journal = "Biomaterials", title = "The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles", volume = "30", number = "12", pages = "2319-2328", doi = "10.1016/j.biomaterials.2009.01.023" }
Misirkić, M. S., Todorović-Marković, B., Vucicevic, L. M., Janjetović, K. D., Jokanović, V. R., Dramićanin, M., Marković, Z. M.,& Trajković, V. S.. (2009). The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles. in Biomaterials, 30(12), 2319-2328. https://doi.org/10.1016/j.biomaterials.2009.01.023
Misirkić MS, Todorović-Marković B, Vucicevic LM, Janjetović KD, Jokanović VR, Dramićanin M, Marković ZM, Trajković VS. The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles. in Biomaterials. 2009;30(12):2319-2328. doi:10.1016/j.biomaterials.2009.01.023 .
Misirkić, Maja S., Todorović-Marković, Biljana, Vucicevic, Ljubica M., Janjetović, Kristina D., Jokanović, Vukoman R., Dramićanin, Miroslav, Marković, Zoran M., Trajković, Vladimir S., "The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles" in Biomaterials, 30, no. 12 (2009):2319-2328, https://doi.org/10.1016/j.biomaterials.2009.01.023 . .