Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus
Abstract
In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.
Keywords:
Mg2+-ATPase / Uterus / Membrane / Metal ions / RatSource:
Environmental Chemistry Letters, 2009, 7, 1, 41-44Funding / projects:
- Signalni putevi delovanja steroidnih hormona i uticaj endogenih i egzogenih faktora na modulaciju procesa u ćelijama sisara (RS-MESTD-MPN2006-2010-143044)
DOI: 10.1007/s10311-007-0132-z
ISSN: 1610-3653
WoS: 000263072800004
Scopus: 2-s2.0-59749096904
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VinčaTY - JOUR AU - Horvat, Anica AU - Milošević, Maja AU - Demajo, Miroslav PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3630 AB - In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity. T2 - Environmental Chemistry Letters T1 - Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus VL - 7 IS - 1 SP - 41 EP - 44 DO - 10.1007/s10311-007-0132-z ER -
@article{ author = "Horvat, Anica and Milošević, Maja and Demajo, Miroslav", year = "2009", abstract = "In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.", journal = "Environmental Chemistry Letters", title = "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus", volume = "7", number = "1", pages = "41-44", doi = "10.1007/s10311-007-0132-z" }
Horvat, A., Milošević, M.,& Demajo, M.. (2009). Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters, 7(1), 41-44. https://doi.org/10.1007/s10311-007-0132-z
Horvat A, Milošević M, Demajo M. Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters. 2009;7(1):41-44. doi:10.1007/s10311-007-0132-z .
Horvat, Anica, Milošević, Maja, Demajo, Miroslav, "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus" in Environmental Chemistry Letters, 7, no. 1 (2009):41-44, https://doi.org/10.1007/s10311-007-0132-z . .