Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients
Nema prikaza
Autori
Radojkovic, M.Ristic, S.
Colovic, M.
Cemerikic-Martinovic, V.
Radojkovic, D.
Krtolica-Žikić, Koviljka
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/...87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance.
Ključne reči:
follicular lymphoma / bcl-2/IgH gene rearrangement / major breakpoint region (MBR) / 5MBR / minor cluster region (mcr)Izvor:
Neoplasma, 2008, 55, 5, 421-427Finansiranje / projekti:
- Serbian Ministry of Science [145061, 143010]
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Radojkovic, M. AU - Ristic, S. AU - Colovic, M. AU - Cemerikic-Martinovic, V. AU - Radojkovic, D. AU - Krtolica-Žikić, Koviljka PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3527 AB - Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance. T2 - Neoplasma T1 - Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients VL - 55 IS - 5 SP - 421 EP - 427 UR - https://hdl.handle.net/21.15107/rcub_vinar_3527 ER -
@article{ author = "Radojkovic, M. and Ristic, S. and Colovic, M. and Cemerikic-Martinovic, V. and Radojkovic, D. and Krtolica-Žikić, Koviljka", year = "2008", abstract = "Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance.", journal = "Neoplasma", title = "Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients", volume = "55", number = "5", pages = "421-427", url = "https://hdl.handle.net/21.15107/rcub_vinar_3527" }
Radojkovic, M., Ristic, S., Colovic, M., Cemerikic-Martinovic, V., Radojkovic, D.,& Krtolica-Žikić, K.. (2008). Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. in Neoplasma, 55(5), 421-427. https://hdl.handle.net/21.15107/rcub_vinar_3527
Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica-Žikić K. Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. in Neoplasma. 2008;55(5):421-427. https://hdl.handle.net/21.15107/rcub_vinar_3527 .
Radojkovic, M., Ristic, S., Colovic, M., Cemerikic-Martinovic, V., Radojkovic, D., Krtolica-Žikić, Koviljka, "Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients" in Neoplasma, 55, no. 5 (2008):421-427, https://hdl.handle.net/21.15107/rcub_vinar_3527 .