Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients
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Radojkovic, M.Ristic, S.
Colovic, M.
Cemerikic-Martinovic, V.
Radojkovic, D.
Krtolica-Žikić, Koviljka
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Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/...87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance.
Keywords:
follicular lymphoma / bcl-2/IgH gene rearrangement / major breakpoint region (MBR) / 5MBR / minor cluster region (mcr)Source:
Neoplasma, 2008, 55, 5, 421-427Funding / projects:
- Serbian Ministry of Science [145061, 143010]
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VinčaTY - JOUR AU - Radojkovic, M. AU - Ristic, S. AU - Colovic, M. AU - Cemerikic-Martinovic, V. AU - Radojkovic, D. AU - Krtolica-Žikić, Koviljka PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3527 AB - Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance. T2 - Neoplasma T1 - Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients VL - 55 IS - 5 SP - 421 EP - 427 UR - https://hdl.handle.net/21.15107/rcub_vinar_3527 ER -
@article{ author = "Radojkovic, M. and Ristic, S. and Colovic, M. and Cemerikic-Martinovic, V. and Radojkovic, D. and Krtolica-Žikić, Koviljka", year = "2008", abstract = "Follicular lymphoma (FL) is characterized by the presence of a t(14; 18) chromosomal translocation that results in overexpression of bcl-2 protein. Bcl-2/IgH gene rearrangement is detected in 80-90% of follicular lymphomas in Western countries. The aim of this study was to analyze the bcl-2/IgH rearrangement in FL lymphoma patients in Serbia, by PCR technique, correlate molecular findings with clinical characteristics and outcome and assess the prognostic significance of these rearrangements. One hundred-seven patients (median age, 54 years; male/female ratio:60/47) diagnosed with FL were included in the study. DNA samples were obtained from paraffin embedded lymphoid tissue of patients. Bcl-2/IgH rearrangement was assessed for the major breakpoint region (MBR), 5MBR and the minor cluster region (mcr) breakpoints by PCR technique. We detected a t(14;18) in 81.3% (87/107) of patients. The distribution of bcl-2-IgH rearrangement was as follows: 88,5% (77/87) in MBR breakpoint, 10,35% (9/87) in 5MBR, whereas mcr bcl-2-IgH rearrangement was observed in one patient (1.15%). No rearrangements were detected in remaining 20 patients (18.7%). This is the first analyses of the frequency of the bcl-2/IgH gene rearrangement in Serbian FL patients, as well as in Eastern European countries. There was no correlation between presence of bcl-2/IgH gene rearrangement and clinical outcome of disease. Incidence of bcl-2/IgH gene rearrangement in Serbian FL patients is relatively high, and similar to frequency in Western countries. Presence of this rearrangement in tumor tissue is not of prognostic significance.", journal = "Neoplasma", title = "Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients", volume = "55", number = "5", pages = "421-427", url = "https://hdl.handle.net/21.15107/rcub_vinar_3527" }
Radojkovic, M., Ristic, S., Colovic, M., Cemerikic-Martinovic, V., Radojkovic, D.,& Krtolica-Žikić, K.. (2008). Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. in Neoplasma, 55(5), 421-427. https://hdl.handle.net/21.15107/rcub_vinar_3527
Radojkovic M, Ristic S, Colovic M, Cemerikic-Martinovic V, Radojkovic D, Krtolica-Žikić K. Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients. in Neoplasma. 2008;55(5):421-427. https://hdl.handle.net/21.15107/rcub_vinar_3527 .
Radojkovic, M., Ristic, S., Colovic, M., Cemerikic-Martinovic, V., Radojkovic, D., Krtolica-Žikić, Koviljka, "Molecular characteristics and prognostic significance of bcl-2/IgH gene rearrangement in Serbian follicular lymphoma patients" in Neoplasma, 55, no. 5 (2008):421-427, https://hdl.handle.net/21.15107/rcub_vinar_3527 .