Modulation of tumor necrosis factor-mediated cell death by fullerenes
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Harhaji, Ljubica M.Isaković, Aleksandra J.
Vucicevic, Ljubica
Janjetović, Kristina D.
Misirkić, Maja
Marković, Zoran M.
Todorović-Marković, Biljana
Nikolić, Nadežda S.
Vranješ-Đurić, Sanja
Nikolić, Zoran M.
Trajković, Vladimir S.
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Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial per...meability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.
Keywords:
apoptosis / fullerene / necrosis / oxidative stress / tumor necrosis factorSource:
Pharmaceutical Research, 2008, 25, 6, 1365-1376
DOI: 10.1007/s11095-007-9486-y
ISSN: 0724-8741
PubMed: 17999162
WoS: 000255753900012
Scopus: 2-s2.0-43149126321
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VinčaTY - JOUR AU - Harhaji, Ljubica M. AU - Isaković, Aleksandra J. AU - Vucicevic, Ljubica AU - Janjetović, Kristina D. AU - Misirkić, Maja AU - Marković, Zoran M. AU - Todorović-Marković, Biljana AU - Nikolić, Nadežda S. AU - Vranješ-Đurić, Sanja AU - Nikolić, Zoran M. AU - Trajković, Vladimir S. PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3445 AB - Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage. T2 - Pharmaceutical Research T1 - Modulation of tumor necrosis factor-mediated cell death by fullerenes VL - 25 IS - 6 SP - 1365 EP - 1376 DO - 10.1007/s11095-007-9486-y ER -
@article{ author = "Harhaji, Ljubica M. and Isaković, Aleksandra J. and Vucicevic, Ljubica and Janjetović, Kristina D. and Misirkić, Maja and Marković, Zoran M. and Todorović-Marković, Biljana and Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Nikolić, Zoran M. and Trajković, Vladimir S.", year = "2008", abstract = "Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.", journal = "Pharmaceutical Research", title = "Modulation of tumor necrosis factor-mediated cell death by fullerenes", volume = "25", number = "6", pages = "1365-1376", doi = "10.1007/s11095-007-9486-y" }
Harhaji, L. M., Isaković, A. J., Vucicevic, L., Janjetović, K. D., Misirkić, M., Marković, Z. M., Todorović-Marković, B., Nikolić, N. S., Vranješ-Đurić, S., Nikolić, Z. M.,& Trajković, V. S.. (2008). Modulation of tumor necrosis factor-mediated cell death by fullerenes. in Pharmaceutical Research, 25(6), 1365-1376. https://doi.org/10.1007/s11095-007-9486-y
Harhaji LM, Isaković AJ, Vucicevic L, Janjetović KD, Misirkić M, Marković ZM, Todorović-Marković B, Nikolić NS, Vranješ-Đurić S, Nikolić ZM, Trajković VS. Modulation of tumor necrosis factor-mediated cell death by fullerenes. in Pharmaceutical Research. 2008;25(6):1365-1376. doi:10.1007/s11095-007-9486-y .
Harhaji, Ljubica M., Isaković, Aleksandra J., Vucicevic, Ljubica, Janjetović, Kristina D., Misirkić, Maja, Marković, Zoran M., Todorović-Marković, Biljana, Nikolić, Nadežda S., Vranješ-Đurić, Sanja, Nikolić, Zoran M., Trajković, Vladimir S., "Modulation of tumor necrosis factor-mediated cell death by fullerenes" in Pharmaceutical Research, 25, no. 6 (2008):1365-1376, https://doi.org/10.1007/s11095-007-9486-y . .