The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase
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Krstić, Danijela Z.Čolović, Mirjana B.
Kralj, M. B.
Trebše, Polonca
Krinulović, Katarina
Vasić, Vesna M.
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The influence of malathion and its four main degradation products found in irradiated solutions (malaoxon, isomalathion, diethyl maleate and O,O-dimethyl phosphate) on acetylcholinesterase (AChE) of free and immobilized bovine erythrocytes was investigated. The concentration-dependent responses to malathion and related organophosphates, malaoxon and isomalathion, of both AChE bioassays used were obtained. The IC (50) values for free and immobilized AChE (3.7 +/- 0.2) x 10(-4) M/(1.6 +/- 0.1) x 10(-4), (2.4 +/- 0.3) x 10(-6)/(3.4 +/- 0.1) x 10(-6) M, and (3.2 +/- 0.3) x 10(-6) M/(2.7 +/- 0.2) x 10(-6) M were obtained in the presence of malathion, malaoxon and isomalathion, respectively. However, diethyl maleate inhibited AChE activity at concentrations GT = 10 mM, while O,O-dimethyl phosphate did not noticeably affect enzyme activity at all investigated concentrations. The relation between the structure of the compounds and their ability to inhibit enzyme activity was discussed.
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Russian Journal of Physical Chemistry A, 2008, 82, 4, 663-668Collections
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VinčaTY - JOUR AU - Krstić, Danijela Z. AU - Čolović, Mirjana B. AU - Kralj, M. B. AU - Trebše, Polonca AU - Krinulović, Katarina AU - Vasić, Vesna M. PY - 2008 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3421 AB - The influence of malathion and its four main degradation products found in irradiated solutions (malaoxon, isomalathion, diethyl maleate and O,O-dimethyl phosphate) on acetylcholinesterase (AChE) of free and immobilized bovine erythrocytes was investigated. The concentration-dependent responses to malathion and related organophosphates, malaoxon and isomalathion, of both AChE bioassays used were obtained. The IC (50) values for free and immobilized AChE (3.7 +/- 0.2) x 10(-4) M/(1.6 +/- 0.1) x 10(-4), (2.4 +/- 0.3) x 10(-6)/(3.4 +/- 0.1) x 10(-6) M, and (3.2 +/- 0.3) x 10(-6) M/(2.7 +/- 0.2) x 10(-6) M were obtained in the presence of malathion, malaoxon and isomalathion, respectively. However, diethyl maleate inhibited AChE activity at concentrations GT = 10 mM, while O,O-dimethyl phosphate did not noticeably affect enzyme activity at all investigated concentrations. The relation between the structure of the compounds and their ability to inhibit enzyme activity was discussed. T2 - Russian Journal of Physical Chemistry A T1 - The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase VL - 82 IS - 4 SP - 663 EP - 668 DO - 10.1134/S0036024408040274 ER -
@article{ author = "Krstić, Danijela Z. and Čolović, Mirjana B. and Kralj, M. B. and Trebše, Polonca and Krinulović, Katarina and Vasić, Vesna M.", year = "2008", abstract = "The influence of malathion and its four main degradation products found in irradiated solutions (malaoxon, isomalathion, diethyl maleate and O,O-dimethyl phosphate) on acetylcholinesterase (AChE) of free and immobilized bovine erythrocytes was investigated. The concentration-dependent responses to malathion and related organophosphates, malaoxon and isomalathion, of both AChE bioassays used were obtained. The IC (50) values for free and immobilized AChE (3.7 +/- 0.2) x 10(-4) M/(1.6 +/- 0.1) x 10(-4), (2.4 +/- 0.3) x 10(-6)/(3.4 +/- 0.1) x 10(-6) M, and (3.2 +/- 0.3) x 10(-6) M/(2.7 +/- 0.2) x 10(-6) M were obtained in the presence of malathion, malaoxon and isomalathion, respectively. However, diethyl maleate inhibited AChE activity at concentrations GT = 10 mM, while O,O-dimethyl phosphate did not noticeably affect enzyme activity at all investigated concentrations. The relation between the structure of the compounds and their ability to inhibit enzyme activity was discussed.", journal = "Russian Journal of Physical Chemistry A", title = "The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase", volume = "82", number = "4", pages = "663-668", doi = "10.1134/S0036024408040274" }
Krstić, D. Z., Čolović, M. B., Kralj, M. B., Trebše, P., Krinulović, K.,& Vasić, V. M.. (2008). The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase. in Russian Journal of Physical Chemistry A, 82(4), 663-668. https://doi.org/10.1134/S0036024408040274
Krstić DZ, Čolović MB, Kralj MB, Trebše P, Krinulović K, Vasić VM. The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase. in Russian Journal of Physical Chemistry A. 2008;82(4):663-668. doi:10.1134/S0036024408040274 .
Krstić, Danijela Z., Čolović, Mirjana B., Kralj, M. B., Trebše, Polonca, Krinulović, Katarina, Vasić, Vesna M., "The influence of malathion and its decomposition products on free and immobilized acetylcholinesterase" in Russian Journal of Physical Chemistry A, 82, no. 4 (2008):663-668, https://doi.org/10.1134/S0036024408040274 . .