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Comethylation of p16 and MGMT genes in colorectal carcinoma: Correlation with clinicopathological features and prognostic value
dc.creator | Krtolica-Žikić, Koviljka | |
dc.creator | Krajnović, Milena M. | |
dc.creator | Usaj-Knežević, Slavica | |
dc.creator | Babić, Dragan | |
dc.creator | Jovanovic, Dusan | |
dc.creator | Dimitrijević, Bogomir B. | |
dc.date.accessioned | 2018-03-01T20:03:14Z | |
dc.date.available | 2018-03-01T20:03:14Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1007-9327 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/3184 | |
dc.description.abstract | AIM: To investigate the significance of p16 and O-6- methylguanine-DNA methyltransferase (MGMT) genes promoter hypermethylation and K-ras mutations on colorectal tumorigenesis and progression. METHODS: p16 and MGMT methylation status was examined on 47 tumor samples, and K-ras mutational status was examined on 85 tumor samples. For methylation analysis, a methylation specific PCR (MS-PCR) method was used. RESULTS: p16 and MGMT promoter methylation was found in 51% (24/47) and 43% (20/47) of CRCS, respectively, and the K-ras mutation was found in 44% (37/85) of CRCs. Comethylation of p16 and MGMT genes was significantly associated with lower aggressiveness of the disease within a two-year period of observation. Only 27% of patients with simultaneous p16 and MGMT methylation showed the detectible occurrence of metastasis and/or death, compared to 67% of patients without double methylation or with no methylation (3/11 vs 22/33, P LT 0.05, chi(2)-test). In addition, p16 and MGMT comethylation showed a trend toward an association with longer survival in patients with CRCs (35.5 +/- 6.0 mo vs 23.1 +/- 3.2 mo, P = 0.072, Log-rank test). Progression of the disease within a two-year period was observed in 66% of patients carrying the K-ras mutation, compared to only 19% of patients with wild type K-ras (29/44 vs 7/37, P LT 0.001, chi(2)-test). The presence of the K-ras mutation significantly correlated to shortened overall survival (20.0 +/- 1.9 mo vs 37.0 +/- 1.8 mo, P LT 0.001, Log-rank test). The comethylation of p16 and MGMT genes was significantly associated with lower aggressiveness of the disease even when K-ras mutations were included in the analysis as an independent variable. CONCLUSION: Our data suggest that comethylation of promoters of p16 and MGMT genes could have a prognostic value in patients with CRC. Specifically, concurrent methylation of both genes correlates with better prognosis. (C) 2007 The WJG Press. All rights reserved. | en |
dc.rights | openAccess | en |
dc.source | World Journal of Gastroenterology | en |
dc.subject | colorectal carcinoma | en |
dc.subject | DNA methylation | en |
dc.subject | p16 | en |
dc.subject | MGMT | en |
dc.subject | K-ras mutation | en |
dc.title | Comethylation of p16 and MGMT genes in colorectal carcinoma: Correlation with clinicopathological features and prognostic value | en |
dc.type | article | en |
dcterms.abstract | Кртолица, Ковиљка; Усај-Кнезевиц, Славица; Бабиц, Драган; Јовановиц, Дусан; Крајновић Милена; Димитријевић Богомир Б.; | |
dc.citation.volume | 13 | |
dc.citation.issue | 8 | |
dc.citation.spage | 1187 | |
dc.citation.epage | 1194 | |
dc.identifier.wos | 000245577500007 | |
dc.identifier.doi | 10.3748/wjg.v13.i8.1187 | |
dc.identifier.pmid | 17451198 | |
dc.identifier.scopus | 2-s2.0-34247129670 |
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