Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats
Apstrakt
Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia exp...ress TH as well as VMAT2 but that social isolation stress does not change their protein levels.
Ključne reči:
Social isolation / rats / intrinsic cardiac ganglia / tyrosine hydroxylase / vesicular monoamine transporter 2Izvor:
Archives of Biological Sciences, 2014, 66, 4, 1645-1651Finansiranje / projekti:
DOI: 10.2298/ABS1404645J
ISSN: 0354-4664; 1821-4339
WoS: 000346477900042
Scopus: 2-s2.0-84921652042
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Jovanović, Predrag AU - Puškaš, Nela AU - Stefanović, Bojana AU - Spasojević, Nataša AU - Dronjak, Slađana PY - 2014 UR - https://vinar.vin.bg.ac.rs/handle/123456789/300 AB - Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels. T2 - Archives of Biological Sciences T1 - Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats VL - 66 IS - 4 SP - 1645 EP - 1651 DO - 10.2298/ABS1404645J ER -
@article{ author = "Jovanović, Predrag and Puškaš, Nela and Stefanović, Bojana and Spasojević, Nataša and Dronjak, Slađana", year = "2014", abstract = "Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels.", journal = "Archives of Biological Sciences", title = "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats", volume = "66", number = "4", pages = "1645-1651", doi = "10.2298/ABS1404645J" }
Jovanović, P., Puškaš, N., Stefanović, B., Spasojević, N.,& Dronjak, S.. (2014). Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences, 66(4), 1645-1651. https://doi.org/10.2298/ABS1404645J
Jovanović P, Puškaš N, Stefanović B, Spasojević N, Dronjak S. Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences. 2014;66(4):1645-1651. doi:10.2298/ABS1404645J .
Jovanović, Predrag, Puškaš, Nela, Stefanović, Bojana, Spasojević, Nataša, Dronjak, Slađana, "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats" in Archives of Biological Sciences, 66, no. 4 (2014):1645-1651, https://doi.org/10.2298/ABS1404645J . .