Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay
Апстракт
A simple Na,K-ATPase assay is described as a suitable method for testing of digoxin photodegradation. The exposure of Na,K-ATPase to the photodegraded samples exhibited reduced inhibition of the enzyme, compared to the unirradiated samples containing equal initial concentrations of drug. The degree of inhibition was dependent on the irradiation time. The concentrations of digoxin in irradiated samples were evaluated by HPLC analysis. Excellent agreement of the results obtained by both methods was observed. The investigation of the influence of irradiated samples on Na,K-ATPase inhibition revealed no side products acting as Na,K-ATPase inhibitors. The cytokinesis block micronucleus test (CBMN) was applied in order to investigate the cytotoxicity of the possible degradation products after exposure to UV irradiation. The results confirmed that the photochemical treatment did not induce the cytotoxic side products. Zero order kinetics, which was observed for digoxin photodegradation and th...e associated reaction mechanism are also discussed. (c) 2005 Elsevier B.V. All rights reserved.
Кључне речи:
digoxin / photochemical degradation / Na,K-ATPase activity / HPLC / genotoxicityИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 2006, 40, 2, 404-409
DOI: 10.1016/j.jpba.2005.05.018
ISSN: 0731-7085
PubMed: 16384681
WoS: 000235319100024
Scopus: 2-s2.0-31344439461
Колекције
Институција/група
VinčaTY - JOUR AU - Vasić, Vesna M. AU - Černigoj, Urh AU - Krinulović, Katarina AU - Joksić, Gordana AU - Franko, Mladen PY - 2006 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2972 AB - A simple Na,K-ATPase assay is described as a suitable method for testing of digoxin photodegradation. The exposure of Na,K-ATPase to the photodegraded samples exhibited reduced inhibition of the enzyme, compared to the unirradiated samples containing equal initial concentrations of drug. The degree of inhibition was dependent on the irradiation time. The concentrations of digoxin in irradiated samples were evaluated by HPLC analysis. Excellent agreement of the results obtained by both methods was observed. The investigation of the influence of irradiated samples on Na,K-ATPase inhibition revealed no side products acting as Na,K-ATPase inhibitors. The cytokinesis block micronucleus test (CBMN) was applied in order to investigate the cytotoxicity of the possible degradation products after exposure to UV irradiation. The results confirmed that the photochemical treatment did not induce the cytotoxic side products. Zero order kinetics, which was observed for digoxin photodegradation and the associated reaction mechanism are also discussed. (c) 2005 Elsevier B.V. All rights reserved. T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay VL - 40 IS - 2 SP - 404 EP - 409 DO - 10.1016/j.jpba.2005.05.018 ER -
@article{ author = "Vasić, Vesna M. and Černigoj, Urh and Krinulović, Katarina and Joksić, Gordana and Franko, Mladen", year = "2006", abstract = "A simple Na,K-ATPase assay is described as a suitable method for testing of digoxin photodegradation. The exposure of Na,K-ATPase to the photodegraded samples exhibited reduced inhibition of the enzyme, compared to the unirradiated samples containing equal initial concentrations of drug. The degree of inhibition was dependent on the irradiation time. The concentrations of digoxin in irradiated samples were evaluated by HPLC analysis. Excellent agreement of the results obtained by both methods was observed. The investigation of the influence of irradiated samples on Na,K-ATPase inhibition revealed no side products acting as Na,K-ATPase inhibitors. The cytokinesis block micronucleus test (CBMN) was applied in order to investigate the cytotoxicity of the possible degradation products after exposure to UV irradiation. The results confirmed that the photochemical treatment did not induce the cytotoxic side products. Zero order kinetics, which was observed for digoxin photodegradation and the associated reaction mechanism are also discussed. (c) 2005 Elsevier B.V. All rights reserved.", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay", volume = "40", number = "2", pages = "404-409", doi = "10.1016/j.jpba.2005.05.018" }
Vasić, V. M., Černigoj, U., Krinulović, K., Joksić, G.,& Franko, M.. (2006). Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay. in Journal of Pharmaceutical and Biomedical Analysis, 40(2), 404-409. https://doi.org/10.1016/j.jpba.2005.05.018
Vasić VM, Černigoj U, Krinulović K, Joksić G, Franko M. Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay. in Journal of Pharmaceutical and Biomedical Analysis. 2006;40(2):404-409. doi:10.1016/j.jpba.2005.05.018 .
Vasić, Vesna M., Černigoj, Urh, Krinulović, Katarina, Joksić, Gordana, Franko, Mladen, "Evaluation of photochemical degradation of digoxin by Na,K-ATPase assay" in Journal of Pharmaceutical and Biomedical Analysis, 40, no. 2 (2006):404-409, https://doi.org/10.1016/j.jpba.2005.05.018 . .