AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR
Apstrakt
Angiotensin II type 1 receptor (AT1) and angiotensin II type 2 receptor (AT2) genes have been investigated in recent years as potential etiologic candidates for cardiovascular and renal diseases. The pathogenic implications of AT1 A1166C and AT2 A1332G gene polymorphisms have been shown. Here we describe a rapid and reliable method for detecting both AT1 and AT2 gene polymorphisms by a single-tube PCR, to reduce analysis time and simplify the genotyping procedure. In contrast to previously described methods, our method does not require hybridization, primer extension, or nested PCR for genetyping. In most previous studies concerning gene polymorphisms of RAS, both AT1 and AT2 receptor gene polymorphisms were investigated. The advantage of our method is that it makes it possible to detect both of these polymorphisms in a duplex PCR. The procedure described is convenient for routine laboratory use with manual sample processing, and offers the potential for further automation as well. Its... simplicity makes it practical for large-scale screening of individuals and families at risk for cardiovascular or renal diseases. (c) 2005 Wiley-Liss, Inc.
Ključne reči:
angiotensin II receptors / polymorphisms / duplex PCRIzvor:
Journal of Clinical Laboratory Analysis, 2005, 19, 2, 84-86
DOI: 10.1002/jcla.20058
ISSN: 0887-8013
PubMed: 15756705
WoS: 000227852100006
Scopus: 2-s2.0-15244361367
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Živković, Maja AU - Stanković, Aleksandra AU - Alavantić, Dragan PY - 2005 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2872 AB - Angiotensin II type 1 receptor (AT1) and angiotensin II type 2 receptor (AT2) genes have been investigated in recent years as potential etiologic candidates for cardiovascular and renal diseases. The pathogenic implications of AT1 A1166C and AT2 A1332G gene polymorphisms have been shown. Here we describe a rapid and reliable method for detecting both AT1 and AT2 gene polymorphisms by a single-tube PCR, to reduce analysis time and simplify the genotyping procedure. In contrast to previously described methods, our method does not require hybridization, primer extension, or nested PCR for genetyping. In most previous studies concerning gene polymorphisms of RAS, both AT1 and AT2 receptor gene polymorphisms were investigated. The advantage of our method is that it makes it possible to detect both of these polymorphisms in a duplex PCR. The procedure described is convenient for routine laboratory use with manual sample processing, and offers the potential for further automation as well. Its simplicity makes it practical for large-scale screening of individuals and families at risk for cardiovascular or renal diseases. (c) 2005 Wiley-Liss, Inc. T2 - Journal of Clinical Laboratory Analysis T1 - AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR VL - 19 IS - 2 SP - 84 EP - 86 DO - 10.1002/jcla.20058 ER -
@article{ author = "Živković, Maja and Stanković, Aleksandra and Alavantić, Dragan", year = "2005", abstract = "Angiotensin II type 1 receptor (AT1) and angiotensin II type 2 receptor (AT2) genes have been investigated in recent years as potential etiologic candidates for cardiovascular and renal diseases. The pathogenic implications of AT1 A1166C and AT2 A1332G gene polymorphisms have been shown. Here we describe a rapid and reliable method for detecting both AT1 and AT2 gene polymorphisms by a single-tube PCR, to reduce analysis time and simplify the genotyping procedure. In contrast to previously described methods, our method does not require hybridization, primer extension, or nested PCR for genetyping. In most previous studies concerning gene polymorphisms of RAS, both AT1 and AT2 receptor gene polymorphisms were investigated. The advantage of our method is that it makes it possible to detect both of these polymorphisms in a duplex PCR. The procedure described is convenient for routine laboratory use with manual sample processing, and offers the potential for further automation as well. Its simplicity makes it practical for large-scale screening of individuals and families at risk for cardiovascular or renal diseases. (c) 2005 Wiley-Liss, Inc.", journal = "Journal of Clinical Laboratory Analysis", title = "AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR", volume = "19", number = "2", pages = "84-86", doi = "10.1002/jcla.20058" }
Živković, M., Stanković, A.,& Alavantić, D.. (2005). AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR. in Journal of Clinical Laboratory Analysis, 19(2), 84-86. https://doi.org/10.1002/jcla.20058
Živković M, Stanković A, Alavantić D. AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR. in Journal of Clinical Laboratory Analysis. 2005;19(2):84-86. doi:10.1002/jcla.20058 .
Živković, Maja, Stanković, Aleksandra, Alavantić, Dragan, "AT1 receptor A1166C and AT2 receptor-1332A/G gene polymorphisms: Efficient genotyping by single-tube PCR" in Journal of Clinical Laboratory Analysis, 19, no. 2 (2005):84-86, https://doi.org/10.1002/jcla.20058 . .