Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex
Апстракт
In the present study the developmental! profile of ATP-hydrolyzing activity promoted by NTPDase 1, its kinetic proper-ties and the enzyme protein abundance associated with synaptic plasma membrane from rat cerebral cortex were characterized. NTPDase I activity increased from birth to day 30; afterwards it decreased and remained unchanged from adulthood (90 days) to senescence (365 days). Kinetic analysis revealed that enzyme exhibited the highest specific activity at day 30 and highest apparent affinity for ATP at day 365; however, V-max/K-m values remained unchanged for each age studied. Immunoblot analysis demonstrated that relative abundance of NTPDase 1 is highest at day 15 during ontogeny. The discrepancy between maximum enzyme activity and maximum enzyme protein abundance indicates that NTPDase 1 may have an additional role,during development. (C) 2004 ISDN. Published by Elsevier Ltd. All rights reserved.
Кључне речи:
cerebral cortex / NTPDase 1 / developmental profile / synaptic plasma membranesИзвор:
International Journal of Developmental Neuroscience, 2005, 23, 1, 45-51
DOI: 10.1016/j.ijdevneu.2004.09.001
ISSN: 0736-5748; 1873-474X
PubMed: 15730886
WoS: 000227752500006
Scopus: 2-s2.0-13844316016
Колекције
Институција/група
VinčaTY - JOUR AU - Nedeljković, N. AU - Banjac, Ana AU - Horvat, Anica AU - Stojiljković, Mirjana AU - Nikezić, Gordana S. PY - 2005 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2870 AB - In the present study the developmental! profile of ATP-hydrolyzing activity promoted by NTPDase 1, its kinetic proper-ties and the enzyme protein abundance associated with synaptic plasma membrane from rat cerebral cortex were characterized. NTPDase I activity increased from birth to day 30; afterwards it decreased and remained unchanged from adulthood (90 days) to senescence (365 days). Kinetic analysis revealed that enzyme exhibited the highest specific activity at day 30 and highest apparent affinity for ATP at day 365; however, V-max/K-m values remained unchanged for each age studied. Immunoblot analysis demonstrated that relative abundance of NTPDase 1 is highest at day 15 during ontogeny. The discrepancy between maximum enzyme activity and maximum enzyme protein abundance indicates that NTPDase 1 may have an additional role,during development. (C) 2004 ISDN. Published by Elsevier Ltd. All rights reserved. T2 - International Journal of Developmental Neuroscience T1 - Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex VL - 23 IS - 1 SP - 45 EP - 51 DO - 10.1016/j.ijdevneu.2004.09.001 ER -
@article{ author = "Nedeljković, N. and Banjac, Ana and Horvat, Anica and Stojiljković, Mirjana and Nikezić, Gordana S.", year = "2005", abstract = "In the present study the developmental! profile of ATP-hydrolyzing activity promoted by NTPDase 1, its kinetic proper-ties and the enzyme protein abundance associated with synaptic plasma membrane from rat cerebral cortex were characterized. NTPDase I activity increased from birth to day 30; afterwards it decreased and remained unchanged from adulthood (90 days) to senescence (365 days). Kinetic analysis revealed that enzyme exhibited the highest specific activity at day 30 and highest apparent affinity for ATP at day 365; however, V-max/K-m values remained unchanged for each age studied. Immunoblot analysis demonstrated that relative abundance of NTPDase 1 is highest at day 15 during ontogeny. The discrepancy between maximum enzyme activity and maximum enzyme protein abundance indicates that NTPDase 1 may have an additional role,during development. (C) 2004 ISDN. Published by Elsevier Ltd. All rights reserved.", journal = "International Journal of Developmental Neuroscience", title = "Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex", volume = "23", number = "1", pages = "45-51", doi = "10.1016/j.ijdevneu.2004.09.001" }
Nedeljković, N., Banjac, A., Horvat, A., Stojiljković, M.,& Nikezić, G. S.. (2005). Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex. in International Journal of Developmental Neuroscience, 23(1), 45-51. https://doi.org/10.1016/j.ijdevneu.2004.09.001
Nedeljković N, Banjac A, Horvat A, Stojiljković M, Nikezić GS. Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex. in International Journal of Developmental Neuroscience. 2005;23(1):45-51. doi:10.1016/j.ijdevneu.2004.09.001 .
Nedeljković, N., Banjac, Ana, Horvat, Anica, Stojiljković, Mirjana, Nikezić, Gordana S., "Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex" in International Journal of Developmental Neuroscience, 23, no. 1 (2005):45-51, https://doi.org/10.1016/j.ijdevneu.2004.09.001 . .