Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain
Nema prikaza
Autori
Terzić, NVujčić, Miroslava T.
Ristić-Fira, Aleksandra
Krstić-Demonacos, Marija
Milanovic, D
Kanazir, Dušan T.
Ruždijić, Sabera
Članak u časopisu
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX ...treatment in an age-specific manner.
Izvor:
Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 2003, 58, 4, 297-303Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Terzić, N AU - Vujčić, Miroslava T. AU - Ristić-Fira, Aleksandra AU - Krstić-Demonacos, Marija AU - Milanovic, D AU - Kanazir, Dušan T. AU - Ruždijić, Sabera PY - 2003 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2623 AB - The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner. T2 - Journals of Gerontology. Series A: Biological Sciences and Medical Sciences T1 - Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain VL - 58 IS - 4 SP - 297 EP - 303 UR - https://hdl.handle.net/21.15107/rcub_vinar_2623 ER -
@article{ author = "Terzić, N and Vujčić, Miroslava T. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Milanovic, D and Kanazir, Dušan T. and Ruždijić, Sabera", year = "2003", abstract = "The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.", journal = "Journals of Gerontology. Series A: Biological Sciences and Medical Sciences", title = "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain", volume = "58", number = "4", pages = "297-303", url = "https://hdl.handle.net/21.15107/rcub_vinar_2623" }
Terzić, N., Vujčić, M. T., Ristić-Fira, A., Krstić-Demonacos, M., Milanovic, D., Kanazir, D. T.,& Ruždijić, S.. (2003). Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58(4), 297-303. https://hdl.handle.net/21.15107/rcub_vinar_2623
Terzić N, Vujčić MT, Ristić-Fira A, Krstić-Demonacos M, Milanovic D, Kanazir DT, Ruždijić S. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences. 2003;58(4):297-303. https://hdl.handle.net/21.15107/rcub_vinar_2623 .
Terzić, N, Vujčić, Miroslava T., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Milanovic, D, Kanazir, Dušan T., Ruždijić, Sabera, "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain" in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58, no. 4 (2003):297-303, https://hdl.handle.net/21.15107/rcub_vinar_2623 .