Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat
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Аутори
Velickovic, NatasaĐorđević, Ana D.
Drakulić, Dunja R.
Stanojević, Ivana
Šećerov, Bojana Lj.
Horvat, Anica
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Glucocorticoids, essential for normal,hypothalamic-pituitary-adrenal (HPA) axis activity, exert their action on the hippocampus through two types of corticosteroid receptors: the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR). Recent studies report that exposure of juvenile rats to cranial irradiation adversely affects HPA axis stability leading to its activation along with radiation-induced inflammation. This study was aimed to examine the acute effects of radiation on HPA axis activity and hippocampal corticosteroid receptor expression in 18-day-old rats. Since immobilization was part of irradiation procedure, both irradiated and sham-irradiated animals were exposed to this unavoidable stress. Our results demonstrate that the irradiated rats exhibited different pattern of corticosteroid receptor expression and hormone levels compared to respective controls. These differences included upregulation of GR protein in the hippocampus with a concomitant elevation of G...R mRNA and an increase in circulating level of corticosterone. In addition, the expression of MR, both at the level of protein and gene expression, was not altered. Taken together, this study demonstrates that cranial irradiation in juvenile rats leads to enhanced HPA axis activity and increased relative GR/MR ratio in hippocampus. The present paper intends to show that neuroendocrine response of normal brain tissue to localized irradiation comprise both activation of HPA axis and altered corticosteroid receptor balance, probably as consequence of innate immune activation.
Кључне речи:
HPA axis / Corticosteroid receptor / Irradiation / HippocampusИзвор:
General Physiology and Biophysics, 2009, 28, SI, 219-227Финансирање / пројекти:
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
Колекције
Институција/група
VinčaTY - JOUR AU - Velickovic, Natasa AU - Đorđević, Ana D. AU - Drakulić, Dunja R. AU - Stanojević, Ivana AU - Šećerov, Bojana Lj. AU - Horvat, Anica PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2559 AB - Glucocorticoids, essential for normal,hypothalamic-pituitary-adrenal (HPA) axis activity, exert their action on the hippocampus through two types of corticosteroid receptors: the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR). Recent studies report that exposure of juvenile rats to cranial irradiation adversely affects HPA axis stability leading to its activation along with radiation-induced inflammation. This study was aimed to examine the acute effects of radiation on HPA axis activity and hippocampal corticosteroid receptor expression in 18-day-old rats. Since immobilization was part of irradiation procedure, both irradiated and sham-irradiated animals were exposed to this unavoidable stress. Our results demonstrate that the irradiated rats exhibited different pattern of corticosteroid receptor expression and hormone levels compared to respective controls. These differences included upregulation of GR protein in the hippocampus with a concomitant elevation of GR mRNA and an increase in circulating level of corticosterone. In addition, the expression of MR, both at the level of protein and gene expression, was not altered. Taken together, this study demonstrates that cranial irradiation in juvenile rats leads to enhanced HPA axis activity and increased relative GR/MR ratio in hippocampus. The present paper intends to show that neuroendocrine response of normal brain tissue to localized irradiation comprise both activation of HPA axis and altered corticosteroid receptor balance, probably as consequence of innate immune activation. T2 - General Physiology and Biophysics T1 - Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat VL - 28 IS - SI SP - 219 EP - 227 UR - https://hdl.handle.net/21.15107/rcub_vinar_2559 ER -
@article{ author = "Velickovic, Natasa and Đorđević, Ana D. and Drakulić, Dunja R. and Stanojević, Ivana and Šećerov, Bojana Lj. and Horvat, Anica", year = "2009", abstract = "Glucocorticoids, essential for normal,hypothalamic-pituitary-adrenal (HPA) axis activity, exert their action on the hippocampus through two types of corticosteroid receptors: the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR). Recent studies report that exposure of juvenile rats to cranial irradiation adversely affects HPA axis stability leading to its activation along with radiation-induced inflammation. This study was aimed to examine the acute effects of radiation on HPA axis activity and hippocampal corticosteroid receptor expression in 18-day-old rats. Since immobilization was part of irradiation procedure, both irradiated and sham-irradiated animals were exposed to this unavoidable stress. Our results demonstrate that the irradiated rats exhibited different pattern of corticosteroid receptor expression and hormone levels compared to respective controls. These differences included upregulation of GR protein in the hippocampus with a concomitant elevation of GR mRNA and an increase in circulating level of corticosterone. In addition, the expression of MR, both at the level of protein and gene expression, was not altered. Taken together, this study demonstrates that cranial irradiation in juvenile rats leads to enhanced HPA axis activity and increased relative GR/MR ratio in hippocampus. The present paper intends to show that neuroendocrine response of normal brain tissue to localized irradiation comprise both activation of HPA axis and altered corticosteroid receptor balance, probably as consequence of innate immune activation.", journal = "General Physiology and Biophysics", title = "Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat", volume = "28", number = "SI", pages = "219-227", url = "https://hdl.handle.net/21.15107/rcub_vinar_2559" }
Velickovic, N., Đorđević, A. D., Drakulić, D. R., Stanojević, I., Šećerov, B. Lj.,& Horvat, A.. (2009). Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat. in General Physiology and Biophysics, 28(SI), 219-227. https://hdl.handle.net/21.15107/rcub_vinar_2559
Velickovic N, Đorđević AD, Drakulić DR, Stanojević I, Šećerov BL, Horvat A. Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat. in General Physiology and Biophysics. 2009;28(SI):219-227. https://hdl.handle.net/21.15107/rcub_vinar_2559 .
Velickovic, Natasa, Đorđević, Ana D., Drakulić, Dunja R., Stanojević, Ivana, Šećerov, Bojana Lj., Horvat, Anica, "Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat" in General Physiology and Biophysics, 28, no. SI (2009):219-227, https://hdl.handle.net/21.15107/rcub_vinar_2559 .