Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy
No Thumbnail
Authors
Fani, MVranješ, Sanja
Archimandritis, Spyridon
Potamianos, S
Xanthopoulos, S
Bouziotis, Penelope
Varvarigou, AD
Article
Metadata
Show full item recordAbstract
The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with Sm-153 has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs. (C) 2002 Elsevier Science Ltd. All rights reserved.
Keywords:
Sm-153 / radiolabeled antibody / radiochemical study / radioimmunotherapySource:
Applied Radiation and Isotopes, 2002, 57, 5, 665-674
DOI: 10.1016/S0969-8043(02)00181-1
ISSN: 0969-8043
PubMed: 12433040
WoS: 000178847900009
Scopus: 2-s2.0-0036844250
Collections
Institution/Community
VinčaTY - JOUR AU - Fani, M AU - Vranješ, Sanja AU - Archimandritis, Spyridon AU - Potamianos, S AU - Xanthopoulos, S AU - Bouziotis, Penelope AU - Varvarigou, AD PY - 2002 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2557 AB - The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with Sm-153 has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs. (C) 2002 Elsevier Science Ltd. All rights reserved. T2 - Applied Radiation and Isotopes T1 - Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy VL - 57 IS - 5 SP - 665 EP - 674 DO - 10.1016/S0969-8043(02)00181-1 ER -
@article{ author = "Fani, M and Vranješ, Sanja and Archimandritis, Spyridon and Potamianos, S and Xanthopoulos, S and Bouziotis, Penelope and Varvarigou, AD", year = "2002", abstract = "The labeling of a monoclonal (anti-CEA) and a polyclonal (IgG) antibody with Sm-153 has been investigated, using the bicyclic anhydride of DTPA (cDTPAa) as the chelating agent. The radiochemical study was performed using a combination of radioanalytical techniques (gel filtration, HPLC, ITLC-SG and SDS-PAGE). Optimization of factors affecting labeling (pH, Ab, Ab-DTPA concentration, etc.) leads to a labeling yield higher than 90%. Biodistribution studies in normal mice showed slow blood clearance and high uptake into the liver, kidney and lungs. (C) 2002 Elsevier Science Ltd. All rights reserved.", journal = "Applied Radiation and Isotopes", title = "Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy", volume = "57", number = "5", pages = "665-674", doi = "10.1016/S0969-8043(02)00181-1" }
Fani, M., Vranješ, S., Archimandritis, S., Potamianos, S., Xanthopoulos, S., Bouziotis, P.,& Varvarigou, A.. (2002). Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy. in Applied Radiation and Isotopes, 57(5), 665-674. https://doi.org/10.1016/S0969-8043(02)00181-1
Fani M, Vranješ S, Archimandritis S, Potamianos S, Xanthopoulos S, Bouziotis P, Varvarigou A. Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy. in Applied Radiation and Isotopes. 2002;57(5):665-674. doi:10.1016/S0969-8043(02)00181-1 .
Fani, M, Vranješ, Sanja, Archimandritis, Spyridon, Potamianos, S, Xanthopoulos, S, Bouziotis, Penelope, Varvarigou, AD, "Labeling of monoclonal antibodies with Sm-153 for potential use in radioimmunotherapy" in Applied Radiation and Isotopes, 57, no. 5 (2002):665-674, https://doi.org/10.1016/S0969-8043(02)00181-1 . .