Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1)
Abstract
Screening for polymorphisms in the human type 1 angiotensin II receptor locus (AGTR1) has led to the identification of an A1166C transversion in the Y-untranslated region. This molecular variant, C-1166, has been linked to essential hypertension. We describe here a rapid method for the detection of this point mutation by a simple modification of PCR amplification with allele-specific oligonucleotides (ASO), so as to avoid a hybridization procedure involving either radioactive- or non-radioactive-labeled probes, labeled primers, or restriction typing. The procedure described is convenient for routine clinical laboratory use with manual sample processing and offers the potential for further automation, as well.
Source:
Genetic Testing, 2002, 6, 2, 133-134
DOI: 10.1089/10906570260199401
ISSN: 1090-6576
PubMed: 12215254
WoS: 000177492000011
Scopus: 2-s2.0-0036591557
Institution/Community
VinčaTY - JOUR AU - Stanković, Aleksandra AU - Alavantić, Dragan PY - 2002 UR - https://vinar.vin.bg.ac.rs/handle/123456789/2540 AB - Screening for polymorphisms in the human type 1 angiotensin II receptor locus (AGTR1) has led to the identification of an A1166C transversion in the Y-untranslated region. This molecular variant, C-1166, has been linked to essential hypertension. We describe here a rapid method for the detection of this point mutation by a simple modification of PCR amplification with allele-specific oligonucleotides (ASO), so as to avoid a hybridization procedure involving either radioactive- or non-radioactive-labeled probes, labeled primers, or restriction typing. The procedure described is convenient for routine clinical laboratory use with manual sample processing and offers the potential for further automation, as well. T2 - Genetic Testing T1 - Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1) VL - 6 IS - 2 SP - 133 EP - 134 DO - 10.1089/10906570260199401 ER -
@article{ author = "Stanković, Aleksandra and Alavantić, Dragan", year = "2002", abstract = "Screening for polymorphisms in the human type 1 angiotensin II receptor locus (AGTR1) has led to the identification of an A1166C transversion in the Y-untranslated region. This molecular variant, C-1166, has been linked to essential hypertension. We describe here a rapid method for the detection of this point mutation by a simple modification of PCR amplification with allele-specific oligonucleotides (ASO), so as to avoid a hybridization procedure involving either radioactive- or non-radioactive-labeled probes, labeled primers, or restriction typing. The procedure described is convenient for routine clinical laboratory use with manual sample processing and offers the potential for further automation, as well.", journal = "Genetic Testing", title = "Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1)", volume = "6", number = "2", pages = "133-134", doi = "10.1089/10906570260199401" }
Stanković, A.,& Alavantić, D.. (2002). Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1). in Genetic Testing, 6(2), 133-134. https://doi.org/10.1089/10906570260199401
Stanković A, Alavantić D. Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1). in Genetic Testing. 2002;6(2):133-134. doi:10.1089/10906570260199401 .
Stanković, Aleksandra, Alavantić, Dragan, "Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1)" in Genetic Testing, 6, no. 2 (2002):133-134, https://doi.org/10.1089/10906570260199401 . .